Analytical Data
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Gene name
Phospholipase C
- Application
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Alternative Names
BC_0670; Phosphatidylcholine cholinephosphohydrolase; Phospholipase C
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Species
Others
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Source
E. coli
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Tag
N-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q81HW1
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Expression Region
M1-R283
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Protein Length
Full Length
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Phospholipase C (PLC) is an essential enzyme that plays a critical role in cellular signaling pathways by hydrolyzing phosphatidylinositol 4,5-bisphosphate (PIP2) to generate inositol trisphosphate (IP3) and diacylglycerol (DAG). These two second messengers are pivotal for various physiological processes, including cell proliferation, differentiation, and apoptosis. Given its significance, PLC has garnered considerable attention in biomedical research, particularly in the context of understanding its implications in diseases such as cancer, cardiovascular disorders, and neurological conditions. The recombinant production of PLC proteins has enabled researchers to study their structure-function relationships more thoroughly and to explore their activity in various cellular contexts. The advancements in recombinant DNA technology and protein expression systems have facilitated the generation of active PLC proteins, providing a platform for detailed biochemical assays, structural studies, and potential therapeutic applications. By utilizing these recombinant proteins, researchers aim to elucidate the mechanisms of PLC activation, regulation, and its downstream signaling pathways, contributing to our understanding of cell biology and the development of targeted therapeutic strategies.
