Analytical Data
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Gene name
CDK4-CCND2
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简介
CDK4 protein, acting as a Ser/Thr kinase in the cyclin D-CDK4 complex, critically regulates the G(1)/S transition by phosphorylating and inhibiting RB family members. This activity, specifically hypophosphorylation of RB1 during early G(1) phase, releases E2F and promotes transcription of genes that drive G(1) progression. CDK4-CCND2 Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK4-CCND2, expressed by Sf9 insect cells, with N-His, N-GST, N-Flag labeled tag.
- Application
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Alternative Names
CDK4; cyclin-dependent kinase 4; PSK J3; cell division protein kinase 4; CMM3; PSK-J3; MGC14458;
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Species
Human
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Source
Baculovirus
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Tag
N-His;N-GST;N-Flag
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P11802-1 (M1-E303)&P30279-1
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Expression Region
P11802-1 (M1-E303)&P30279-1 (E2-L289)
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Molecular Weight
60.3 KDa & 36.0 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
CDK4-CCND2 reconstituted protein is a critical focus of study in the field of cell cycle regulation and cancer biology. Cyclin-dependent kinase 4 (CDK4) plays an essential role in the transition from the G1 phase to the S phase of the cell cycle, driving cellular proliferation. Its activity is tightly regulated by cyclins, with cyclin D2 (CCND2) being a key regulatory partner. Abnormalities in the CDK4-CCND2 pathway, such as overexpression or mutations, are frequently observed in various cancers, contributing to uncontrolled cell division and tumorigenesis. The reconstitution of CDK4 with CCND2 provides a valuable experimental system to investigate the molecular mechanisms underlying cell cycle progression and the oncogenic potential of this complex. Researchers employ various biochemical and biophysical techniques to characterize the interactions and functional dynamics of CDK4-CCND2, aiming to elucidate how this signaling axis contributes to cancer development. Furthermore, understanding the therapeutic implications of targeting this pathway offers potential avenues for novel cancer treatments, as inhibiting CDK4 could halt the proliferation of cancerous cells. Insights gained from these studies may pave the way for the development of specific CDK4 inhibitors, which could transform cancer therapy by selectively targeting tumor cells while sparing normal tissues. Thus, the investigation of CDK4-CCND2 reconstituted protein is not only pivotal for comprehending cell cycle regulation but also holds promise for advancing cancer treatment strategies.
