Analytical Data
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基因名
CDK6-CCND1
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简介
The CDK6ic complex is an important serine/threonine protein kinase that regulates cell cycle progression and differentiation, promoting the G1/S transition by phosphorylating substrates such as pRB/RB1 and NPM1. It interacts with D-type G1 cyclins during interphase to form active pRB/RB1 kinase, which controls cell cycle entry. CDK6-CCND1 Heterodimer Protein, Human (sf9, GST, Flag, His) is the recombinant human-derived CDK6-CCND1 Heterodimer, expressed by Sf9 insect cells, with N-Flag, N-His, N-GST labeled tag.
- Application
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别名
CDK6; CCND2; Cyclin-dependent kinase 6; Cell division protein kinase 6; Serine/threonine-protein kinase PLSTIRE; G1/S-specific cyclin-D2
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种属
Human
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表达系统
Baculovirus
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标签
N-Flag;N-His;N-GST
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q00534 (M1-A326)&P24385
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表达区间
Q00534 (M1-A326)&P24385 (M1-I295)
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分子量
63.5 kDa&36.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cyclin-dependent kinase 6 (CDK6) and Cyclin D1 (CCND1) play pivotal roles in regulating the cell cycle and are integral to cellular proliferation. Dysregulation of these proteins is frequently associated with various cancers, making them significant targets for therapeutic interventions. The CDK6-CCND1 complex promotes the transition from the G1 to the S phase of the cell cycle, facilitating DNA synthesis and cell division. Research into the CDK6-CCND1 recombinant protein has gained momentum due to its potential to elucidate molecular mechanisms underlying tumorigenesis. By studying this complex, scientists aim to better understand its role in oncogenesis, the mechanisms of CDK6 and CCND1 overexpression, and their interaction with other cell cycle regulators. Additionally, the development of inhibitors targeting the CDK6-CCND1 pathway has the potential to provide novel strategies for cancer treatment, particularly in tumors exhibiting aberrant activity of this pathway. Overall, the investigation of CDK6-CCND1 recombinant proteins is crucial for advancing our knowledge of cancer biology and developing effective therapies.
