Analytical Data
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Gene name
ASK1
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简介
ASK1 protein is a key serine/threonine kinase in the MAP kinase pathway, coordinating cellular responses to environmental changes and affecting cell fate, differentiation, and survival. ASK1 is a key player in apoptosis and promotes mitochondria-dependent caspase activation. ASK1 Protein, Human (His) is the recombinant human-derived ASK1 protein, expressed by E. coli , with C-6*His labeled tag.
- Application
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Alternative Names
MAP3K5; Mitogen-activated protein kinase kinase kinase 5; Apoptosis signal-regulating kinase 1; ASK-1; MAPK/ERK kinase kinase 5; MEK kinase 5; MEKK 5
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q99683
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Expression Region
M649-K946
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Protein Length
Partial
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Molecular Weight
34.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
ASK1 (Apoptosis Signal-regulating Kinase 1) is a member of the MAPK (mitogen-activated protein kinase) kinase family, and it plays a critical role in cellular stress responses, inflammation, and apoptosis. Dysregulation of ASK1 is implicated in various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases, making it a significant target for therapeutic intervention. The activation of ASK1 is often triggered by oxidative stress and pro-inflammatory cytokines, leading to the activation of downstream signaling pathways such as the JNK (c-Jun N-terminal kinase) and p38 MAPK pathways, which ultimately result in programmed cell death. Research on ASK1 has focused on understanding its molecular mechanisms, identifying potential inhibitors or activators, and exploring its role in specific disease contexts. The development of ASK1-targeted therapies aims to mitigate its pathological effects, offering hope for novel treatments. Moreover, the production of recombinant ASK1 protein has facilitated the study of its biochemical properties and regulatory mechanisms. These recombinant proteins are essential for conducting in vitro assays and structural biology studies, ultimately enhancing our understanding of this kinase's functions and its potential as a drug target. Recent advancements in biotechnological methods have enabled more efficient expression and purification of ASK1, allowing for detailed investigations into its structure-function relationships and interactions with other signaling molecules. Overall, the study of ASK1 and its recombinant forms is crucial for elucidating its role in health and disease, paving the way for innovative therapeutic strategies.
