Analytical Data
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Gene name
EEF2K
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简介
EEF2K, a threonine kinase, critically regulates protein synthesis by modulating peptide chain elongation. Activation by upstream kinases like AMPK or TRPM7 leads to phosphorylation of EEF2, inhibiting its binding to ribosomes and decelerating protein synthesis. EEF2K's intricate control underscores its pivotal role in modulating cellular processes related to protein translation. EEF2K Protein, Human (GST) is the recombinant human-derived EEF2K protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase
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Species
Human
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O00418
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Expression Region
A2-E725
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Protein Length
Full Length of Mature Protein
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Molecular Weight
108.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
EEF2K (Eukaryotic Elongation Factor 2 Kinase) is a crucial enzyme that functions as a key regulator of protein synthesis by phosphorylating eukaryotic elongation factor 2 (eEF2), ultimately inhibiting translation and thereby affecting cellular growth and proliferation. Research into EEF2K has gained significant attention due to its involvement in various physiological and pathological processes, including stress responses, cancer development, and neurodegenerative diseases. Under conditions of cellular stress, EEF2K is activated, leading to a decrease in protein synthesis which conserves energy and resources, and helps cells adapt to adverse conditions. The role of EEF2K in cancer biology is particularly noteworthy, as its overexpression is often associated with enhanced tumor growth and resistance to chemotherapy, making it a potential target for therapeutic intervention. In addition, EEF2K's regulatory functions extend to various signaling pathways, including those related to mTOR and p53, which are critical for maintaining cellular homeostasis. However, the precise mechanisms governing EEF2K’s activity and its interactions with other molecular players in these pathways remain to be thoroughly elucidated. Consequently, ongoing studies focus on characterizing EEF2K's structure and function, exploring its potential as a biomarker for disease, and developing small molecules or inhibitors that can modulate its activity for therapeutic purposes. The growing body of evidence underscores the significance of EEF2K in both basic biological research and clinical applications, paving the way for future investigations into its role in health and disease.
