Analytical Data
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基因名
APAF1
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简介
APAF1 protein is a key apoptotic component that coordinates cytochrome c-dependent caspase-9 precursor activation and initiates caspase-3 activation and apoptosis. APAF1 forms an apoptotic loop with cytochrome c and dATP, interacts with caspase-9 precursor, and releases mature caspase-9. APAF1 Protein, Human (P. pastoris, His) is the recombinant human-derived APAF1 protein, expressed by P. pastoris , with N-6*His labeled tag.
- Application
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别名
APAF 1; Apaf-1; APAF_HUMAN; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413
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种属
Human
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表达系统
P. pastoris
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标签
N-6*His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O14727
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表达区间
S104-L415
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氨基酸序列
SGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDIL
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蛋白长度
Partial
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分子量
37.4 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
APAF1 (apoptosis protease activating factor 1) is a critical component of the apoptosome, a multi-protein complex that plays a pivotal role in the intrinsic pathway of apoptosis. The activation of APAF1 is mediated by cytochrome c released from mitochondria, which then binds to APAF1, leading to its conformational change and oligomerization into a heptameric structure. This process facilitates the recruitment and activation of procaspase-9, ultimately triggering a cascade of caspase activation that results in programmed cell death. Given its central role in apoptosis, dysregulation of APAF1 is implicated in various pathological conditions, including cancer, neurodegenerative diseases, and autoimmune disorders, where evasion of apoptosis contributes to disease progression. Research on recombinant APAF1 protein has gained momentum as it presents a valuable tool for understanding the molecular mechanisms governing apoptosis and for developing potential therapeutic strategies. By producing and characterizing recombinant APAF1, researchers aim to elucidate its structure-function relationships, investigate its interactions with other apoptotic proteins, and explore its regulatory mechanisms. Furthermore, studying APAF1 in vitro and in vivo could lead to novel insights into how target molecules can modulate its activity, potentially paving the way for innovative treatments that restore apoptotic processes in disease contexts. Overall, recombinant APAF1 serves not only as a model for studying apoptosis but also as a potential therapeutic target in cancer and other diseases characterized by dysregulated cell death.
