Analytical Data
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Gene name
PRAK/MAPKAPK5
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简介
PRAK/MAPKAPK5 protein, a serine/threonine kinase, responds to cellular stress and pro-inflammatory cytokines and acts as a tumor suppressor through MAP kinases (including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta) activated by phosphorylation. Initially located in the nucleus, upon activation, it moves to the cytoplasm and phosphorylates the heat shock protein HSP27. PRAK/MAPKAPK5 Protein, Human (sf9, His-GST) is the recombinant human-derived PRAK/MAPKAPK5 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
- Application
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Alternative Names
MAP kinase-activated protein kinase 5; MAPKAP-K5; MK5; PRAK
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Species
Human
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Source
Baculovirus
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Tag
N-GST;N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8IW41-2
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Expression Region
M1-Q471
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Protein Length
Partial
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Molecular Weight
35-45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PRAK (p38 MAPK-activated protein kinase) is a serine/threonine kinase that is primarily activated by the p38 mitogen-activated protein kinase (MAPK) pathway, which plays a crucial role in cellular responses to stress, inflammation, and differentiation. MAPKAPK5, also known as PRAK, is involved in various cellular processes, including cell proliferation, survival, and apoptosis. Research has shown that PRAK/MAPKAPK5 can influence the activity of several transcription factors and signaling pathways, thereby regulating gene expression in response to environmental stimuli. Given its integral role in the MAPK signaling cascade, PRAK has become a focal point in cancer research, as it can modulate tumor growth and metastasis. Understanding the structure and function of PRAK/MAPKAPK5 through the study of recombinant proteins offers valuable insights into its mechanistic role in disease states. This research can also pave the way for the development of therapeutic strategies targeting the MAPK pathway, which is often dysregulated in various cancers. As such, the recombinant expression and characterization of PRAK/MAPKAPK5 is vital for elucidating its biochemical properties, interactions with other cellular proteins, and potential as a therapeutic target in oncology.
