Analytical Data
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Gene name
PR-Set7
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简介
The PR-Set7 Protein methylates Lys-20 of histone H4, repressing gene transcription and aiding cell proliferation and chromatin condensation. It is expressed in multiple tissues, including the prostate (RPKM 17.6), esophagus (RPKM 16.8), and others. PR-Set7 Protein, Human is the recombinant human-derived PR-Set7 protein, expressed by E. coli , with tag free.
- Application
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Biological Activity
Measured in a cell proliferation assay using MDA-MB-231 cells. The ED50 for this effect is 26.51 ng/mL, corresponding to a specific activity is 3.772×104 units/mg. Measured in a cell proliferation assay using MDA-MB-231 cells. The ED50 for this effect is 26.51 ng/mL, corresponding to a specific activity is 3.772×104 units/mg.
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Alternative Names
N-lysine methyltransferase KMT5A; PR-Set7; KMT5A; PRSET7; SET8; SETD8
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Species
Human
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Source
E. coli
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Tag
Tag Free
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
Q9NQR1-2
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Expression Region
K195-H352
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Protein Length
Partial
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Molecular Weight
18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
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Protein Description
PR-Set7, a member of the SET-domain protein family, is a histone methyltransferase that plays a crucial role in regulating gene expression and chromatin dynamics through histone modification. Specifically, PR-Set7 is known for its ability to mono-methylate lysine 20 on histone H4 (H4K20me1), which is integral to various biological processes, including cell cycle progression, DNA repair, and cellular differentiation. The dysregulation of PR-Set7 has been implicated in several diseases, particularly cancer, where altered histone methylation patterns can lead to aberrant gene expression and genomic instability. Researchers are increasingly focused on understanding the mechanisms by which PR-Set7 functions in these contexts, as well as exploring its potential as a therapeutic target. The study of PR-Set7 and its associated pathways not only enhances our comprehension of epigenetic regulation but also provides insights into the molecular underpinnings of tumorigenesis. Efforts to elucidate the precise roles of PR-Set7, including its interactions with other epigenetic regulators and its impact on chromatin structure, continue to be a significant area of research within the field of molecular biology and oncology.
