Analytical Data
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Gene name
SMAD4
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简介
SMAD4 is a class of common mediator SMAD (Co-SMAD), which belongs to the dwarfin/SMAD family. It conducts cell signals through the SMAD4/TGF-β pathway and plays an important role in promoting bone development and tissue homeostasis. SMAD4 induces chondrogenesis of human bone marrow mesenchymal stem cells, and specifically targets stem cells to participate in self-renewal of hematopoietic stem cells. SMAD4 can also protect cell apoptosis and inhibit epithelial cell proliferation, showing antitumor effects[1][2]. SMAD4 Protein, Human (His) has the 552 a.a. sequence (M1-D552), produced in E.coli with C-terminal 6*His-tag.
- Application
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Alternative Names
Mothers Against Decapentaplegic Homolog 4; MAD Homolog 4; Mothers Against DPP Homolog 4; Deletion Target in Pancreatic Carcinoma 4; SMAD Family Member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4
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Species
Human
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Source
E. coli
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Tag
C-6*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13485
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Expression Region
M1-D552
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AA Sequence
MDNMSITNTPTSNDACLSIVHSLMCHRQGGESETFAKRAIESLVKKLKEKKDELDSLITAITTNGAHPSKCVTIQRTLDGRLQVAGRKGFPHVIYARLWRWPDLHKNELKHVKYCQYAFDLKCDSVCVNPYHYERVVSPGIDLSGLTLQSNAPSSMMVKDEYVHDFEGQPSLSTEGHSIQTIQHPPSNRASTETYSTPALLAPSESNATSTANFPNIPVASTSQPASILGGSHSEGLLQIASGPQPGQQQNGFTGQPATYHHNSTTTWTGSRTAPYTPNLPHHQNGHLQHHPPMPPHPGHYWPVHNELAFQPPISNHPAPEYWCSIAYFEMDVQVGETFKVPSSCPIVTVDGYVDPSGGDRFCLGQLSNVHRTEAIERARLHIGKGVQLECKGEGDVWVRCLSDHAVFVQSYYLDREAGRAPGDAVHKIYPSAYIKVFDLRQCHRQMQQQAATAQAAAAAQAAAVAGNIPGPGSVGGIAPAISLSAAAGIGVDDLRRLCILRMSFVKGWGPDYPRQSIKETPCWIEIHLHRALQLLDEVLHTMPIADPQPLD
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Protein Length
Full Length
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Molecular Weight
62 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
SMAD4 is a pivotal protein in the transforming growth factor-beta (TGF-β) signaling pathway, playing a crucial role in mediating cellular responses to TGF-β and related cytokines. It acts as a common mediator for signals that regulate key biological processes, including cell growth, differentiation, and apoptosis. Dysregulation of SMAD4 has been implicated in various human diseases, particularly in cancers such as pancreatic and colorectal tumors, where mutations or deletions of the SMAD4 gene are common. Research into SMAD4 recombinant proteins has gained attention as it allows for the exploration of its structural and functional properties, providing insights into the molecular mechanisms underlying TGF-β signaling. These studies enhance our understanding of cancer biology and could lead to the development of novel therapeutic strategies targeting SMAD4 and its signaling pathways. Moreover, the availability of recombinant SMAD4 proteins facilitates the study of protein interactions, post-translational modifications, and the identification of potential small molecules that can modulate its activity, promising avenues for therapeutic intervention in SMAD4-related malignancies. As the need for targeted cancer therapies intensifies, ongoing research into the characterization and functional analysis of SMAD4 recombinant proteins is vital for uncovering its potential as a biomarker and therapeutic target in clinical oncology.
