Ipodix Biotech: Custom Nanobody Service
Nanobodies (single-domain antibodies or VHH antibodies) exhibit unique advantages in drug development, in vitro diagnostics, and cellular imaging due to their small molecular weight, high stability, and strong tissue penetration. Ipodix Biotech specializes in nanobody preparation services, offering end-to-end solutions from antigen design to antibody optimization through advanced camelid immunization technology and high-throughput screening platforms.
Technical Advantages & Workflow
Nanobodies, derived from the natural heavy-chain antibodies of camelids (e.g., alpacas, llamas), consist of a single variable domain (VHH) and offer unparalleled benefits:
● Small size (~15 kDa): Enables tissue barrier penetration for in vivo imaging or targeted delivery.
● High stability: Resists extreme pH, high temperatures, and proteases, ideal for industrial production.
● Superior solubility: Efficiently expressed in prokaryotic systems without glycosylation.
Ipodix’s Workflow:
| Antigen Design & Immunization | 8–10 weeks | Immunization protocol, serum titer reports. |
| VHH Library Construction | 4–6 weeks | Phage display library, primary screening data. |
| High-Throughput Screening & Optimization | 6–8 weeks | Candidate sequences with affinity validation. |
| Expression & Functional Validation | 4–6 weeks | High-purity antibodies (≥95%), application test data. |
Service Highlights
Diverse Antigen Compatibility
a. Supports proteins, peptides, haptens (e.g., toxins, hormones), and membrane protein targets.
b. Enhances immunization efficacy for challenging antigens (e.g., GPCRs, ion channels) using nanodisc or liposome encapsulation.Efficient Screening Platform
a. Phage display technology constructs VHH libraries (>10^9 diversity) to isolate high-affinity antibodies (KD ≤1 nM).
b. Offers humanization, affinity maturation, and multivalent formats (dimers/trimers).Flexible Production Systems
a. Prokaryotic (E. coli) or eukaryotic (yeast/mammalian) expression for varied purity and activity needs.
b. Multiple tags (His, Fc, Flag) and labeling options (fluorescence, biotinylation).
Applications
| Drug Development | Targeted ADCs for tumor microenvironments. |
| IVD Development | High-sensitivity test strips/microfluidic chips. |
| Cellular Imaging | Real-time tracking of intracellular protein dynamics. |
| Industrial Testing | Rapid detection of food toxins or environmental pollutants. |
| Research Tools | Membrane protein structure analysis, protein interaction studies. |
Quality Assurance & Standards
Ipodix adheres to ISO 9001 standards to ensure reliable performance:
● Purity: SDS-PAGE and HPLC with ≥95% purity.
● Affinity: SPR/BLI validation (KD ≤10 nM).
● Functionality: ELISA, immunohistochemistry, or neutralization data.
● Stability: Testing under 60°C heat, freeze-thaw cycles, and long-term storage (4°C/-20°C).
Collaboration Process
Requirement Submission: Define target, application, and deliverables.
Protocol Customization: Receive technical proposal and quote within 48 hours.
Agreement Finalization: Confirm acceptance criteria and timeline.
Project Execution: Regular updates with experimental progress.
Delivery & Validation: Antibody samples, QC reports, and documentation.
Why Choose Ipodix?
● Proven Expertise: 500+ completed projects covering oncology, infectious diseases, and rare targets.
● Timely Delivery: Standard timeline 18–24 weeks; expedited projects in 14 weeks.
● Post-Service Support: 12 months of free technical consultation and antibody retesting.
Accelerate Your Research with Precision!
Contact the Ipodix technical team via our website or hotline to discuss your project.
