Analytical Data
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基因名
Monoglyceride lipase
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简介
Monoglyceride lipase is a key enzyme in lipid metabolism and plays a key role in converting monoacylglycerols into free fatty acids and glycerol, contributing to important cellular processes. In addition to being involved in lipid metabolism, this enzyme also hydrolyzes the endocannabinoid 2-arachidonoylglycerol, thereby affecting the complex regulation of endocannabinoid signaling, nociception, and pain perception. Monoglyceride lipase Protein, Rat (P.pastoris, His) is the recombinant rat-derived Monoglyceride lipase protein, expressed by P. pastoris , with N-His labeled tag.
- Application
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别名
Mgll; Mgl2; Monoglyceride lipase; MGL; Monoacylglycerol lipase; MAGL
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种属
Rat
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表达系统
P. pastoris
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标签
N-His
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q8R431
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表达区间
M1-P303
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氨基酸序列
MPEASSPRRTPQNVPYQDLPHLVNADGQYLFCRYWKPSGTPKALIFVSHGAGEHCGRYDELAQMLKRLDMLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISILAAAERPTHFSGMILISPLILANPESASTLKVLAAKLLNFVLPNISLGRIDSSVLSRNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHRIAVAGARCLP
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蛋白长度
Full Length
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Monoglyceride lipase (MGL) is an important enzyme that catalyzes the hydrolysis of monoglycerides into glycerol and free fatty acids, playing a crucial role in lipid metabolism. This enzyme is particularly significant in various physiological processes including energy production, membrane structure integrity, and signal transduction. The study of recombinant MGL protein has gained traction due to its potential applications in both fundamental research and industrial processes. Advances in molecular cloning and expression systems, such as bacterial, yeast, and mammalian cells, have enabled the production of high yields of MGL, facilitating detailed biochemical characterization and understanding of its structure-function relationships. Additionally, the significance of MGL in the gastrointestinal tract as well as its implications in obesity, diabetes, and other metabolic disorders underscores the necessity for in-depth investigations into its mechanisms of action. By creating recombinant forms of the enzyme, researchers aim to uncover its enzymatic properties, regulatory mechanisms, and potential inhibitors, which could lead to therapeutic applications. The ability to produce MGL in a controlled environment allows for tailored studies and enhances the prospects of developing dietary supplements or pharmaceuticals aimed at modulating lipid metabolism. Overall, the exploration of recombinant monoglyceride lipase holds great promise for advancing our understanding of lipid biology and for contributing to the development of novel interventions in metabolic diseases.
