Analytical Data
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Gene name
DCK/Deoxycytidine kinase
- Application
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P27707
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Expression Region
Met1~Leu260
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Molecular Weight
34kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Deoxycytidine kinase (DCK) is a crucial enzyme in the salvage pathway of nucleotide metabolism, specifically involved in the phosphorylation of deoxyribonucleosides to their corresponding monophosphates. This enzyme plays a significant role in maintaining the nucleotide pool for DNA synthesis and repair, making it essential for cell proliferation and survival. The study of DCK is particularly relevant in the context of cancer therapy, as it is involved in the activation of several nucleoside analogs used as chemotherapeutic agents, including gemcitabine and cytarabine. Understanding the molecular mechanisms regulating DCK activity can provide insights into drug resistance mechanisms in malignant cells and enhance the efficacy of nucleoside-based therapies. Furthermore, DCK's role in various diseases, including viral infections such as hepatitis and HIV, highlights its potential as a therapeutic target. The recombinant production of DCK allows for detailed biochemical studies, structural analyses, and assessment of its interaction with nucleoside analogs. Advancements in biotechnology have facilitated the optimization of DCK expression systems, enabling the generation of large quantities of purified protein for research purposes. This has opened avenues for exploring DCK's structure-function relationships, post-translational modifications, and interactions with other cellular factors. Overall, the research on recombinant DCK is vital for deciphering its biological significance and potential applications in drug development and disease management.
