Analytical Data
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基因名
Cytosine deaminase/CodA
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简介
Cytosine deaminase (CodA) protein plays a key role in the pyrimidine salvage pathway by catalyzing the hydrolytic deamination of cytosine to uracil. This enzyme activity enables cells to efficiently utilize cytosine for pyrimidine nucleotide synthesis. Cytosine deaminase/CodA Protein, E.coli (His-SUMO) is the recombinant E. coli-derived Cytosine deaminase/CodA protein, expressed by E. coli , with N-His, N-SUMO labeled tag.
- Application
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别名
codA; b0337; JW0328Cytosine deaminase; CD; CDA; CDase; EC 3.5.4.1; Cytosine aminohydrolase; Isoguanine deaminase; EC 3.5.4.-
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种属
E.coli
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表达系统
E. coli
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标签
N-His;N-SUMO
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P25524
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表达区间
S2-R427
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氨基酸序列
SNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR
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蛋白长度
Full Length of Mature Protein
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Cytosine deaminase (CDA), also known as CodA, is an enzyme that catalyzes the deamination of cytosine to uracil, playing a pivotal role in nucleic acid metabolism and the regulation of nucleotide pools in various organisms. The study of CDA has gained significance in the context of cancer therapy, particularly in the development of suicide gene therapies, where the enzyme converts a non-toxic prodrug into a toxic metabolite specifically within tumor cells. This targeted approach enhances the therapeutic efficacy while minimizing systemic toxicity. Additionally, CDA is of interest in synthetic biology, where its ability to manipulate nucleotides can be utilized for creating novel biosynthetic pathways and metabolic engineering. Researchers have focused on characterizing and optimizing CodA through recombinant protein expression techniques, enhancing its stability, activity, and substrate specificity. Such studies involve the cloning of the CDA gene, expression in suitable host organisms, and subsequent purification of the protein. Understanding the structure-function relationships of CDA not only facilitates its application in medical and biotechnological fields but also deepens our comprehension of evolutionary adaptation and enzyme mechanisms. The exploration of CDA and its recombinant forms opens avenues for innovative treatments and biotechnological applications, making it a key focus for ongoing research in enzymology and gene therapy.
