Analytical Data
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Gene name
ATG14
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简介
ATG14 is critical for basal and induced autophagy, determining PI3KC3-C1 localization, helping autophagosome formation and BECN1 translocation. It enhances PIK3C3 activity in a BECN1-dependent manner, which is essential for BECN1 phosphorylation. ATG14 Protein, Human (Myc, His) is the recombinant human-derived ATG14 protein, expressed by E. coli , with C-Myc, N-10*His labeled tag.
- Application
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Alternative Names
4832427M01; ATG14; Atg14L; Autophagy-related protein 14-like protein; Barkor; Beclin 1-associated autophagy-related key regulator; D14Ertd114e
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Species
Human
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Source
E. coli
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Tag
C-Myc;N-10*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6ZNE5-1
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Expression Region
M1-R492
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AA Sequence
MASPSGKGARALEAPGCGPRPLARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSRLKSKQEEFQKEVLKAMEGKWITDQLRWKIMSCKMRIEQLKQTICKGNEEMEKNSEGLLKTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLRSHYERLANLRRSHILELTSVIFPIEEVKTGVRDPADVSSESDSAMTSSTVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYSWVEEKKTTQGPDMEQSNPAYTISAALCYATQLVNILSHILDVNLPKKLCNSEFCGENLSKQKFTRAVKKLNANILYLCFSQHVNLDQLQPLHTLRNLMYLVSPSSEHLGRSGPFEVRADLEESMEFVDPGVAGESDESGDERVSDEETDLGTDWENLPSPRFCDIPSQSVEVSQSQSTQASPPIASSSAGGMISSAAASVTSWFKAYTGHR
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Protein Length
Full Length of Isoform-1
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Molecular Weight
70 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The study of ATG14, a pivotal protein in the autophagy process, has gained significant attention due to its crucial role in regulating cellular degradation pathways. Autophagy is an essential cellular mechanism that maintains homeostasis by degrading damaged organelles and proteins, especially under stress conditions or nutrient deprivation. ATG14 is a key component of the class III phosphatidylinositol 3-kinase (PI3K) complex, which is vital for the formation of autophagosomes—structures that encapsulate and degrade cellular debris. Research indicates that ATG14 not only facilitates the nucleation of autophagic membranes but also interacts with various other proteins that influence autophagy's initiation and progression. Furthermore, dysregulation of autophagy is linked to several pathological conditions, including neurodegenerative diseases, cancer, and infections, highlighting the importance of ATG14 as a potential therapeutic target. Understanding the molecular mechanisms of ATG14 and its interactions within the autophagic pathway can provide insights into potential interventions for diseases that arise from autophagy dysfunction. Recent advances in recombinant protein technology have facilitated the production and study of ATG14, allowing for detailed investigations of its structure, function, and the impact of its post-translational modifications. Such research endeavors aim to elucidate the complexities of autophagy regulation and its broader implications for cellular health and disease management.
