Analytical Data
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Gene name
Coagulation Factor III/F3
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简介
Coagulation Factor III (F3), or Tissue Factor (TF), initiates blood coagulation by forming a complex with circulating factor VII or VIIa. The [TF:VIIa] complex critically activates factors IX or X through limited proteolysis, initiating the coagulation cascade on the cell surface. F3's interaction with HSPE, inhibited by heparin, promotes the generation of activated factor X, emphasizing its central role in regulating hemostatic processes. Coagulation Factor III/F3 Protein, Canine (HEK293, His) is the recombinant canine-derived Coagulation Factor III/F3 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Alternative Names
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA
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Species
Canine
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Source
HEK293
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Tag
C-8*His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q4W6L5
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Expression Region
T35-E248
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Protein Length
Partial
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Molecular Weight
35-45 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Coagulation Factor III, also known as tissue factor (TF), is a vital protein that initiates the coagulation cascade upon vascular injury. It plays a crucial role in hemostasis and is essential for normal blood clot formation. The significance of TF extends beyond coagulation, as it is also implicated in various pathological conditions, including thrombosis, cancer progression, and inflammation. Due to its pivotal role, researchers have focused on characterizing and developing recombinant forms of Coagulation Factor III for therapeutic applications. Recombinant TF can serve as a valuable tool in understanding coagulation mechanisms, and its application may provide insights into designing novel anticoagulant therapies. Moreover, studies on recombinant TF are instrumental in investigating its potential use as a biomarker for certain diseases and as a target for new treatment strategies, thereby making it a subject of extensive research in the fields of hematology and oncology. The advancement of recombinant technology has paved the way for producing high-quality TF, enabling scientists to explore its functions and interactions in greater detail, ultimately contributing to better management of coagulation-related disorders.
