Analytical Data
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Gene name
CTCF
- Application
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Alternative Names
Brother of the regulator of imprinted sites CCCTC-binding factor CTCF paralog CTCF-like protein
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Species
Mouse
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Source
Yeast
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Tag
N- His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
A2APF3
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Expression Region
1-636aa
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Molecular Weight
75.1 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
CTCF (CCCTC-binding factor) is a multifunctional zinc finger domain protein that plays a crucial role in the regulation of gene expression, chromatin organization, and the formation of higher-order genomic structures. Identified as a key player in the maintenance of genome integrity, CTCF acts as an insulator to block aberrant enhancer-promoter interactions, thereby ensuring proper transcriptional control. Its binding sites are significantly enriched at the boundaries of topologically associating domains (TADs), which are fundamental units of spatial genome organization. Additionally, CTCF is involved in various biological processes, including cellular differentiation, development, and response to environmental stimuli. Abnormalities in CTCF function and expression have been linked to a range of diseases, including cancer, highlighting its importance in normal cellular physiology. Recent advances in high-throughput sequencing and chromatin conformation capture techniques have provided deeper insights into the dynamic roles of CTCF in shaping the genome architecture and its interactions with other proteins and nucleic acids. As research continues to unravel the complex mechanisms through which CTCF influences gene regulation and chromatin structure, it is becoming increasingly evident that this protein is a central hub in the intricate network governing cellular function and identity. Understanding CTCF's multifaceted roles may pave the way for novel therapeutic strategies targeting gene dysregulation and other associated pathologies.
