Analytical Data
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基因名
SOST
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简介
SOST proteins act as potent inhibitors of bone growth by effectively inhibiting Wnt signaling and subsequent bone formation. SOST exerts its inhibitory effect by interacting with key Wnt pathway components, including LRP4, LRP5, and LRP6. SOST Protein, Mouse (HEK293, His) is the recombinant mouse-derived SOST protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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生物活性
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 1.080 μg/mL in the presence of 20 ng/mL Recombinant Human Wnt-3a, corresponding to a specific activity is 925.9259 units/mg. Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 1.080 μg/mL in the presence of 20 ng/mL Recombinant Human Wnt-3a, corresponding to a specific activity is 925.9259 units/mg.
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别名
Sclerostin; Sost
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种属
Mouse
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
AAK13455.1
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表达区间
Q24-Y211
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氨基酸序列
QGWQAFRNDATEVIPGLGEYPEPPPENNQTMNRAENGGRPPHHPYDAKDVSEYSCRELHYTRFLTDGPCRSAKPVTELVCSGQCGPARLLPNAIGRVKWWRPNGPDFRCIPDRYRAQRVQLLCPGGAAPRSRKVRLVASCKCKRLTRFHNQSELKDFGPETARPQKGRKPRPGARGAKANQAELENAY
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蛋白长度
Partial
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分子量
32.0 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SOST (Sclerostin) is a glycoprotein primarily produced by osteocytes, playing a crucial role in bone metabolism and homeostasis. Its primary function is to inhibit the Wnt signaling pathway, which is essential for osteoblast differentiation and bone formation. Elevated SOST expression is associated with conditions such as osteoporosis, where bone density is significantly reduced, leading to an increased risk of fractures. Conversely, decreased SOST levels have been linked to diseases characterized by excessive bone formation, like osteopetrosis. Given these implications, researchers have been investigating the therapeutic potential of SOST and its analogs in treating bone-related disorders. Techniques such as recombinant protein expression and purification have been employed to produce SOST for in vitro and in vivo studies, aiming to better understand its mechanisms and develop potential SOST-targeted therapies. Furthermore, studies are exploring the effects of SOST inhibition on enhancing bone density and improving overall skeletal health, positioning SOST as a significant target for future osteoporosis treatments.
