Analytical Data
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基因名
GM-CSF R alpha
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简介
GM-CSF R alpha is the alpha subunit of the heterodimeric receptor for colony stimulating factor 2. GM-CSF R alpha binds to the cytokine with high specificity and low affinity[1]. GM-CSF R alpha binds to GM-CSF and induces cell differentiation[2]. GM-CSF R alpha monoclonal antibody decreases GM-CSFRα expression on GM-CSF-responsive cells and shows anti-inflammatory activity in rheumatoid arthritis (RA)[3]. GM-CSF R alpha Protein, Human (HEK293, His) is a recombinant protein with a C-Terminal His label, It consists of 298 amino acids (E23-G320) and is produced in HEK293 cells.
- Application
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生物活性
1. Measured by its ability to inhibit GM-CSF-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is 0.5-2.509 μg/mL. 2. Loaded Mavrilimumab (HY-P99031) on AHC2 biosensor, can bind GM-CSF R alpha Protein, Human (HEK293, His) with an affinity constant of 7.867E-10 M as determined in BLI assay. Measured by its ability to inhibit GM-CSF-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is 2.509 μg/mL in the presence of 40 pg/mL of rhGM-CSF. corresponding to a specific activity is 398.5652 units/mg. Loaded Mavrilimumab (HY-P99031) on AHC2 biosensor, can bind GM-CSF R alpha Protein, Human (HEK293, His) with an affinity constant of 7.867E-10 M as determined in BLI assay.
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别名
rHuCD116, His; Granulocyte-Macrophage Colony-Stimulating Factor Receptor Subunit Alpha; CD116; CSF2RA; CSF2R; CSF2RY
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
P15509
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表达区间
E23-G320
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氨基酸序列
EKSDLRTVAPASSLNVRFDSRTMNLSWDCQENTTFSKCFLTDKKNRVVEPRLSNNECSCTFREICLHEGVTFEVHVNTSQRGFQQKLLYPNSGREGTAAQNFSCFIYNADLMNCTWARGPTAPRDVQYFLYIRNSKRRREIRCPYYIQDSGTHVGCHLDNLSGLTSRNYFLVNGTSREIGIQFFDSLLDTKKIERFNPPSNVTVRCNTTHCLVRWKQPRTYQKLSYLDFQYQLDVHRKNTQPGTENLLINVSGDLENRYNFPSSEPRAKHSVKIRAADVRILNWSSWSEAIEFGSDDGHHHHHH
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蛋白长度
Extracellular Domain
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分子量
54-80 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Granulocyte-macrophage colony-stimulating factor receptor alpha (GM-CSF R alpha) is a critical component of the immune system, playing a pivotal role in hematopoiesis and the activation of immune cells such as neutrophils and macrophages. Research into GM-CSF R alpha has gained momentum due to its implications in various clinical conditions, including autoimmune diseases, cancer, and infectious diseases. The receptor, which binds GM-CSF, is integral to mediating its biological effects, including cell proliferation, differentiation, and survival. Understanding the signaling pathways and mechanisms of action related to GM-CSF R alpha is crucial for developing targeted therapies. Recombinant GM-CSF R alpha proteins have been produced to study these interactions in vitro and in vivo, providing insights into their functional roles and potential as therapeutic targets. The development of GM-CSF R alpha as a recombinant protein allows researchers to explore its structure-function relationships and its influence on immune responses, which may lead to novel interventions to modulate the immune system in various pathologies. Additionally, the characterization of GM-CSF R alpha can facilitate the design of antagonists or agonists that could fine-tune its activity, providing a promising avenue for both basic research and clinical applications. Overall, the ongoing studies on GM-CSF R alpha are essential for unraveling its complex biology and harnessing its potential for therapeutic benefits.
