Analytical Data
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Gene name
PFDN1
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简介
PFDN1 Protein is pivotal in protein folding, binding specifically to cytosolic chaperonin (c-CPN) and aiding the transfer of target proteins. It also binds nascent polypeptide chains, facilitating their folding amidst diverse alternative pathways. Structurally, PFDN1 forms a heterohexamer with two PFD-alpha type and four PFD-beta type subunits. PFDN1 Protein, Human (His) is the recombinant human-derived PFDN1 protein, expressed by E. coli , with N-His labeled tag.
- Application
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Alternative Names
Prefoldin subunit 1; PFDN1; PFD1
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O60925
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Expression Region
M1-Q122
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Protein Length
Full Length
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Molecular Weight
17 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
PFDN1, or Prefoldin 1, is a crucial molecular chaperone involved in the protein folding process within the cytosol. It plays a significant role in assisting the proper folding of newly synthesized proteins, thus preventing misfolding and aggregation that can lead to cellular stress and diseases. PFDN1 is part of the prefoldin complex, which facilitates the transfer of substrates to the cytosolic chaperonin TRIC (TCP1 Ring Complex), enabling efficient protein folding. Recent studies have highlighted its importance not only in maintaining proteostasis but also in cellular processes like cell differentiation and stress responses. The dysfunction of PFDN1 has been associated with various pathological conditions, including neurodegenerative diseases and cancer. Given its pivotal role in cellular homeostasis, PFDN1 recombinant protein has garnered attention for both research and therapeutic purposes. By exploring the structural and functional properties of PFDN1 through recombinant techniques, researchers aim to uncover its mechanisms in protein homeostasis and investigate its potential as a biomarker or therapeutic target in diseases related to protein misfolding. Understanding PFDN1's interactions with other chaperones and substrates can illuminate new avenues for drug development and intervention strategies in diseases characterized by proteostasis disruption. Thus, the study of PFDN1 and its recombinant forms presents significant potential for enhancing our comprehension of cellular protein management and addressing diseases associated with protein misfolding.
