Analytical Data
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Gene name
14-3-3 beta
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简介
14-3-3 beta proteins serve as adapter proteins that regulate multiple signaling pathways by recognizing phosphoserine or phosphothreonine motifs and regulating chaperone activity. It acts as a negative regulator during osteogenesis, inhibiting nuclear translocation of SRPK2 and preventing neuronal apoptosis. 14-3-3 beta Protein, Human (GST) is the recombinant human-derived 14-3-3 beta protein, expressed by E. coli , with N-GST labeled tag.
- Application
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Alternative Names
KCIP-1; 14-3-3 protein beta/alpha; GW128; Protein 1054; YWHAB
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Species
Human
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Source
E. coli
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Tag
N-GST
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P31946
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Expression Region
M1-N246
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Protein Length
Full Length
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Molecular Weight
52 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The 14-3-3 protein family consists of a set of highly conserved regulatory proteins that play crucial roles in various cellular processes, including signal transduction, cell cycle control, and apoptosis. Among these, the 14-3-3 beta isoform has garnered attention due to its involvement in key physiological functions and its potential implications in diseases, such as cancer and neurodegenerative disorders. Research on 14-3-3 beta has highlighted its capacity to interact with a wide range of partner proteins, influencing their stability, localization, and function. Notably, 14-3-3 beta is implicated in modulating pathways related to cell growth and survival, making it a significant player in oncogenic signaling. The dysregulation of 14-3-3 proteins, including the beta isoform, has been linked to various pathologies, underscoring the importance of understanding their molecular mechanisms. Additionally, the study of 14-3-3 beta offers insights into therapeutic targets for drug development, as manipulating its interactions could provide novel strategies for treating diseases where this protein is perturbed. As research continues to unfold the complexities surrounding 14-3-3 beta, its role in cellular homeostasis and pathology remains a vibrant avenue of investigation, pointing toward its potential as a biomarker and target in precision medicine.
