Analytical Data
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Gene name
AGO3
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简介
The AGO3 protein is critical for RNA-mediated gene silencing (RNAi) by binding to short RNAs such as microRNAs (miRNAs) and inhibiting the translation of complementary mRNAs. It is involved in stabilizing small RNA derivatives (siRNA) produced by Alu repeats and the DR2 retinoic acid response element (RARE) transcribed by processed RNA polymerase III in stem cells. AGO3 Protein, Human (sf9, His) is the recombinant human-derived AGO3 protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Application
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Alternative Names
Protein argonaute-3; hAgo3; eIF-2C 3; Argonaute-3; EIF2C3
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Species
Human
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Source
Baculovirus
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9H9G7
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Expression Region
M1-A860
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Protein Length
Full Length
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Molecular Weight
90 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
AGO3 (Argonaute 3) is a member of the Argonaute protein family that plays a crucial role in the RNA interference (RNAi) pathway, which is essential for regulating gene expression and maintaining genome integrity. As a key component of the RNA-induced silencing complex (RISC), AGO3 interacts with small RNA molecules, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), to guide the complex to target mRNAs for degradation or translational repression. Research has revealed that AGO3 is involved in various biological processes, including development, differentiation, and response to viral infections. Given its importance in gene silencing mechanisms, AGO3 is a significant focus in studies aimed at understanding the underlying mechanisms of gene regulation and their implications in diseases, including cancer and viral infections. Moreover, the ability to manipulate AGO3 and its associated pathways holds potential for the development of RNA-based therapeutic strategies. However, there are still gaps in understanding the structural and functional characteristics of AGO3, as its interactions with different small RNAs and target mRNAs may vary widely across different contexts. Consequently, ongoing studies are exploring the reconstitution of AGO3 with various RNA molecules to elucidate its functional dynamics, thereby providing insights that could lead to innovative approaches in biotechnology and medicine.
