Analytical Data
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基因名
GAS6
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简介
The GAS6 protein is a ligand for AXL, TYRO3 and MER receptors and affects a variety of cellular processes.GAS6/AXL signaling promotes endothelial cell survival, aids cytokine signaling in natural killer cell development, supports liver regeneration, affects gonadotropin-releasing hormone neuron survival and migration, modulates platelet activation, and regulates thrombotic responses.GAS6 Protein, Human (HEK293, C-His) is the recombinant human-derived GAS6 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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生物活性
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 15-50 ng/mL. Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 27.66 ng/mL. Corresponding to a specific activity is 3.6×104 U/mg.
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别名
AXLLG; AXLLGAXL stimulatory factor; AXSFAXL receptor tyrosine kinase ligand; Gas6; GAS-6; growth arrest-specific 6; growth arrest-specific protein 6
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种属
Human
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表达系统
HEK293
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标签
C-6*His
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纯度
Greater than 95% as determined by SDS-PAGE.
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蛋白编号
Q14393-2
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表达区间
A31-A678
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氨基酸序列
ALLPAREATQFLRPRQRRAFQVFEEAKQGHLERECVEELCSREEAREVFENDPETDYFYPRYLDCINKYGSPYTKNSGFATCVQNLPDQCTPNPCDRKGTQACQDLMGNFFCLCKAGWGGRLCDKDVNECSQENGGCLQICHNKPGSFHCSCHSGFELSSDGRTCQDIDECADSEACGEARCKNLPGSYSCLCDEGFAYSSQEKACRDVDECLQGRCEQVCVNSPGSYTCHCDGRGGLKLSQDMDTCEDILPCVPFSVAKSVKSLYLGRMFSGTPVIRLRFKRLQPTRLVAEFDFRTFDPEGILLFAGGHQDSTWIVLALRAGRLELQLRYNGVGRVTSSGPVINHGMWQTISVEELARNLVIKVNRDAVMKIAVAGDLFQPERGLYHLNLTVGGIPFHEKDLVQPINPRLDGCMRSWNWLNGEDTTIQETVKVNTRMQCFSVTERGSFYPGSGFAFYSLDYMRTPLDVGTESTWEVEVVAHIRPAADTGVLFALWAPDLRAVPLSVALVDYHSTKKLKKQLVVLAVEHTALALMEIKVCDGQEHVVTVSLRDGEATLEVDGTRGQSEVSAAQLQERLAVLERHLRSPVLTFAGGLPDVPVTSAPVTAFYRGCMTLEVNRRLLDLDEAAYKHSDITAHSCPPVEPAAA
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蛋白长度
Full Length of Isoform-2 Mature Protein
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分子量
75-95 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Gas6 (Growth Arrest-Specific Gene 6) is a vitamin K-dependent protein that plays a crucial role in various biological processes, including cell survival, proliferation, and immune regulation. It has garnered significant attention due to its involvement in several pathophysiological conditions, notably cancer, cardiovascular diseases, and autoimmune disorders. Gas6 interacts with receptor tyrosine kinases such as Axl, Tyro3, and Mer, which are implicated in the regulation of cell growth and apoptosis. Research has demonstrated that Gas6 can enhance the survival of various cell types under stress conditions, including apoptosis triggered by chemotherapy. Additionally, elevated levels of Gas6 have been correlated with poor prognosis in certain cancers, highlighting its potential as a biomarker and therapeutic target. Various studies have focused on the recombinant expression of Gas6 to elucidate its structural and functional properties, paving the way for the development of Gas6-based therapies and diagnostic tools. Understanding its mechanism of action and refining its recombinant production are critical steps toward harnessing its therapeutic potential in treating diseases where Gas6 signaling is dysregulated. The ongoing exploration of GAS6 recombinant proteins in preclinical and clinical settings represents a promising frontier in biomedical research, aiming to translate these findings into effective therapeutic strategies.
