Analytical Data
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Gene name
sFRP-3
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简介
The sFRP-3 protein is a soluble Frizzled-related protein that modulates Wnt signaling by directly interacting with Wnt, thereby regulating cell growth and differentiation. Known as FRZB, it acts as a Wnt8 signaling antagonist with crucial effects on limb skeletogenesis. sFRP-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived sFRP-3 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
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Biological Activity
Measured by its ability to inhibit proliferation in MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.797 µg/mL. Measured by its ability to inhibit proliferation of MC3T3-E1. The ED50 for this effect is 5.797μg/mL, corresponding to a specific activity is 172.503 units/mg.
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Species
Mouse
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by SDS-PAGE.
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Uniprot
P97401
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Expression Region
A33-S323
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AA Sequence
AACEPVRIPLCKSLPWNMTKMPNHLHHSTQANAILAMEQFEGLLGTHCSPDLLFFLCAMYAPICTIDFQHEPIKPCKSVCERARQGCEPILIKYRHSWPESLACDELPVYDRGVCISPEAIVTADGADFPMDSSTGHCRGASSERCKCKPVRATQKTYFRNNYNYVIRAKVKEVKMKCHDVTAVVEVKEILKASLVNIPRDTVNLYTTSGCLCPPLTVNEEYVIMGYEDEERSRLLLVEGSIAEKWKDRLGKKVKRWDMKLRHLGLGKTDASDSTQNQKSGRNSNPRPARS
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Protein Length
Full Length of Mature Protein
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Molecular Weight
35-37 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
sFRP-3 (secreted frizzled-related protein 3) is a member of the sFRP family, which plays a crucial role in modulating Wnt signaling pathways involved in various biological processes, including embryonic development, cell differentiation, and tissue homeostasis. The dysregulation of sFRP-3 has been implicated in several diseases, particularly in cancer, where it may act as a tumor suppressor or promoter, depending on the context. Researchers have focused on understanding the structure and function of sFRP-3 to elucidate its mechanisms in disease progression and potential therapeutic applications. Studies have shown that sFRP-3 can influence cell proliferation and apoptosis by interacting with Wnt ligands, which are key players in cell signaling. The recombinant expression of sFRP-3 has become a valuable tool for investigating its biological functions and interactions. Additionally, the exploration of sFRP-3's role in regulating stem cell niches provides insights into its potentials in regenerative medicine. Overall, the research on sFRP-3 recombinant proteins aims to deepen our understanding of its molecular mechanisms in health and disease, offering avenues for novel therapeutic strategies.
