Analytical Data
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Gene name
FGL1
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简介
The FGL1 protein has signaling receptor binding activity and negatively regulates T cell activation and immune response. It is involved in adipose tissue development, cholesterol metabolism, and response to stilbenoid. FGL1 is located in the extracellular region and is orthologous to the human FGL1 gene. It is primarily expressed in the liver at the E18 stage. FGL1 Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived FGL1 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
Immobilized Mouse LAG-3, hFc Tag (HY-P73843) at 5 μg/mL (100 μL/well) can bind Mouse FGL1. The ED50 for this effect is 0.5343 μg/mL, corresponding to a specific activity is 1.87×103 Unit/mg. Immobilized Mouse LAG-3, hFc Tag (HY-P73843) at 5 μg/mL (100 μL/well) can bind Mouse FGL1. The ED50 for this effect is 0.5343 μg/mL, corresponding to a specific activity is 1.87×103 Unit/mg.
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Alternative Names
Fibrinogen-like protein 1; FGL1; HP-041; Hepassocin; HFREP-1; LFIRE-1
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Species
Mouse
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
NP_663569.2
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Expression Region
L23-I314
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AA Sequence
LESENCLREQVRLRAQVHQLETRVKQQQTMIAQLLHEKEVQFLDKGSENSFIDLGGKKQYADCSEIYNDGFKQSGFYKIKPLQSLAEFSVYCDMSDGGGWTVIQRRSDGSENFNRGWNDYENGFGNFVQNNGEYWLGNKNINLLTIQGDYTLKIDLTDFEKNSSFAQYQSFKVGDKKSFYELNIGEYSGTAGDSLSGTFHPEVQWWASHQRMKFSTWDRDNDNYQGNCAEEEQSGWWFNRCHSANLNGVYYRGSYRAETDNGVVWYTWHGWWYSLKSVVMKIRPSDFIPNII
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Protein Length
Full Length of Mature Protein
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Molecular Weight
60.08 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
FGL1, or Fibrinogen-like protein 1, is a member of the fibrinogen-related protein family that plays a crucial role in various biological processes, including immunoregulation, inflammation, and tumor progression. It has garnered significant attention in recent years due to its dual function in modulating both immune responses and tumor microenvironments. Elevated FGL1 expression has been correlated with poor prognosis in several types of cancer, suggesting its potential as a biomarker for disease progression and a target for therapeutic intervention. Research has shown that FGL1 can inhibit T cell activation and promote immune evasion by tumors, highlighting its role in the suppression of anti-tumor immunity. Moreover, FGL1 has been implicated in facilitating tumor growth and metastatic spread. Consequently, the development of FGL1 recombinant proteins aims to elucidate its mechanisms of action, assess its functional characteristics, and explore its potential in clinical applications, particularly in oncology and immunotherapy. By gaining a deeper understanding of FGL1's role in immune modulation and tumor dynamics, researchers hope to harness its properties for novel therapeutic strategies, enhancing the efficacy of existing treatments and improving patient outcomes. This background lays the foundation for ongoing studies focusing on FGL1's interactions within the immune system, its structural and functional properties, and the feasibility of targeting FGL1 in therapeutic contexts.
