Analytical Data
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Gene name
Caspase-1/CASP1
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简介
Caspase-1/CASP1 subunit p10 protein is a thiol protease complex involved in inflammation by catalyzing the cleavage of IL1B, IL18, and Gasdermin-D (GSDMD). It activates a pro-inflammatory response, releases mature cytokines IL1B and IL18, and initiates pyroptosis through cleavage of GSDMD. Caspase-1/CASP1 Protein, Human (Active, T388A, His) is the recombinant human-derived Caspase-1/CASP1, expressed by E. coli, with N-His labeled tag.
- Application
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Species
Human
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Source
E. coli
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Tag
N-His
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P29466-1
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Expression Region
P29466-1 (N120-D297&A317-H404, T388A)
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Protein Length
Partial
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Molecular Weight
32.0 KDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Caspase-1 (CASP1) is a vital protease that plays a pivotal role in the inflammatory response and the regulation of apoptosis. It is crucial for the activation of pro-inflammatory cytokines, such as interleukin-1β (IL-1β) and interleukin-18 (IL-18), through a process known as pyroptosis. Dysregulation of CASP1 activity is associated with various inflammatory diseases, including autoimmune disorders, neurodegenerative diseases, and cancer. The recombinant CASP1 protein has been a focus of research for its potential therapeutic applications, particularly in understanding its activation mechanisms and developing targeted therapies against inflammatory conditions. Through the production of recombinant CASP1, researchers can investigate its enzymatic activity, regulatory pathways, and interaction with other cellular components. Moreover, the study of recombinant CASP1 provides insights into its structural properties, aiding in the design of inhibitors that could modulate its activity in disease contexts. Given the increasing recognition of the role of inflammation in numerous diseases, the recombinant CASP1 protein serves as an essential tool in both basic and applied research, paving the way for novel therapeutic strategies to manage inflammatory diseases effectively.
