Analytical Data
-
Gene name
Siglec-5
-
简介
Siglec-5 Protein is a member of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. Siglec-5 is a member of the CD33-related subset of Siglecs and is expressed on myeloid cells of the hemopoietic system. Siglec-5 inhibits the response of innate immune cells, such as monocytes and neutrophils against pathogens. Siglec-5 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Siglec-5 protein, expressed by HEK293 , with C-6*His labeled tag.
- Application
-
Alternative Names
Sialic acid-binding Ig-like lectin 5; Siglec-5
-
Species
Cynomolgus
-
Source
HEK293
-
Tag
C-6*His
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
A0A0B4J1D1
-
Expression Region
E17-G435
-
AA Sequence
EEPGYELQVQKSVTVQEGLCVLVPCSFSYPWNSWYSPSPLYVYWFRDGESQYYAEAVATNNPDRRVKPETQGRFRLLGDVRNKNCSLSIGDARMGDTGNYYFRVERGRNVKYNYVQNKLNLEVTALTEKPDVRFLEPLESGRLTRLSCSLPGSCEVGRPLTFSWTGDVLSPLDPETTGSSELTLTPRPEDHGTNLTCHVKHQGAQVTTERTVQLNVSYAPQNITIFRNGTALEILQNTSTLLVLEGEALRLLCEAPSNPPAHLSWFQASSAPNTTPIANTGILELPRVEFTKEGVFTCRAQHPLGSLHIFLNLSVYSLPQLLGPSCSWEAESLHCSCSFRAWPAPSLCWWLGEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLTSVLKVSCKGWNTYGSQSGSVVLLQGRLNLRTG
-
Protein Length
Partial
-
Molecular Weight
90 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Siglec-5 is a member of the sialic acid-binding immunoglobulin-like lectins (Siglecs) family, which play crucial roles in cell signaling and immune regulation. Research has shown that Siglec-5 is predominantly expressed on immune cells, such as monocytes and macrophages, and is involved in modulating immune responses, particularly in the context of inflammation and pathogen recognition. Understanding the function and mechanisms of Siglec-5 is critical for elucidating its role in various immunological disorders, including autoimmune diseases and infections. Recent studies have indicated that Siglec-5 can interact with sialylated glycoproteins, affecting cellular communication and immune evasion strategies employed by pathogens. Furthermore, the development of recombinant Siglec-5 proteins allows for in-depth exploration of its biological activity and could aid in the development of novel therapeutic strategies aimed at targeting specific immune pathways. Enhanced knowledge of Siglec-5 interactions and signaling pathways may reveal potential implications for treating diseases characterized by immune dysregulation. Overall, ongoing research involving Siglec-5 recombinant proteins is pivotal for advancing our understanding of immune regulation and discovering new avenues for clinical interventions.
