Analytical Data
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基因名
Siglec-5
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简介
Siglec-5 Protein, a putative adhesion molecule, participates in sialic-acid dependent cellular binding, showing equal affinity for alpha-2,3-linked and alpha-2,6-linked sialic acid. Its sialic acid recognition site may be masked through cis interactions with sialic acids on the same cell surface. Siglec-5 Protein, Human (HEK293, Fc) is the recombinant human-derived Siglec-5 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. Immobilized Siglec-5 at 1 μg/mL (100 μL/well) can bind Anti Siglec-5 antibody. The ED50 for this effect is 0.7241 μg/mL. Measured by its binding ability in a functional ELISA. Immobilized Siglec-5 at 1 μg/mL (100 μL/well) can bind Anti Siglec-5 antibody. The ED50 for this effect is 0.7241 μg/mL.
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别名
Sialic acid-binding Ig-like lectin 5; Siglec-5; CD33 antigen-like 2; Obesity-binding protein 2; OB-BP2; CD170
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种属
Human
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表达系统
HEK293
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标签
C-hFc
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
O15389
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表达区间
E17-T434
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氨基酸序列
EKPVYELQVQKSVTVQEGLCVLVPCSFSYPWRSWYSSPPLYVYWFRDGEIPYYAEVVATNNPDRRVKPETQGRFRLLGDVQKKNCSLSIGDARMEDTGSYFFRVERGRDVKYSYQQNKLNLEVTALIEKPDIHFLEPLESGRPTRLSCSLPGSCEAGPPLTFSWTGNALSPLDPETTRSSELTLTPRPEDHGTNLTCQMKRQGAQVTTERTVQLNVSYAPQTITIFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQSGSVLLLQGRSNLGT
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蛋白长度
Partial
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分子量
90-120 kDa.
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Siglec-5 is a member of the sialic acid-binding immunoglobulin-like lectins (Siglecs) family, which play crucial roles in cell signaling and immune regulation. Research has shown that Siglec-5 is predominantly expressed on immune cells, such as monocytes and macrophages, and is involved in modulating immune responses, particularly in the context of inflammation and pathogen recognition. Understanding the function and mechanisms of Siglec-5 is critical for elucidating its role in various immunological disorders, including autoimmune diseases and infections. Recent studies have indicated that Siglec-5 can interact with sialylated glycoproteins, affecting cellular communication and immune evasion strategies employed by pathogens. Furthermore, the development of recombinant Siglec-5 proteins allows for in-depth exploration of its biological activity and could aid in the development of novel therapeutic strategies aimed at targeting specific immune pathways. Enhanced knowledge of Siglec-5 interactions and signaling pathways may reveal potential implications for treating diseases characterized by immune dysregulation. Overall, ongoing research involving Siglec-5 recombinant proteins is pivotal for advancing our understanding of immune regulation and discovering new avenues for clinical interventions.
