Analytical Data
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Gene name
FGFR-1
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简介
FGFR-1 protein is a tyrosine-protein kinase that acts as a cell surface receptor for fibroblast growth factor and plays a critical role in the complex regulation of embryonic development, cell proliferation, differentiation, and migration. FGFR-1 mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1, MAP kinase signaling pathways, and AKT1 signaling pathways. FGFR-1 Protein, Rhesus Macaque (HEK293, His) is the recombinant Rhesus Macaque-derived FGFR-1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.3610ng/mL, corresponding to a specific activity is 2.770×106 units/mg. Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.3610ng/mL, corresponding to a specific activity is 2.770×106 units/mg.
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Alternative Names
Fibroblast growth factor receptor 1; FGFR-1; BFGFR; FLT-2; CD331; HBGFR
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Species
Rhesus Macaque
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Source
HEK293
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Tag
C-6*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
H9FRD4
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Expression Region
R22-Y283
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AA Sequence
RPSPTLPEQDALPSSEDDDDDDDSSSEEKETDNTKPNPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLY
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Protein Length
Partial
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Molecular Weight
50-75 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fibroblast growth factor receptor 1 (FGFR-1) is a critical receptor in cell signaling pathways that regulate various biological processes, including cell proliferation, differentiation, and survival. Aberrations in FGFR-1 signaling are implicated in numerous diseases, particularly various cancers, where its overexpression or mutations can lead to tumorigenesis. Research on FGFR-1 has gained momentum due to its potential as a therapeutic target; inhibiting its activity may offer new avenues for cancer treatment. Recombinant FGFR-1 proteins are produced for functional studies to elucidate the receptor's mechanism of action and its interactions with ligands and downstream signaling molecules. Such studies are crucial for developing FGFR-1 inhibitors and understanding their efficacy in cancer therapy. Additionally, the characterization of FGFR-1 in different contexts, such as in the presence of tumor microenvironment factors, is essential for interpreting its role in disease progression. Through recombinant protein studies, researchers aim to develop selective antagonists and explore combination therapies to enhance treatment outcomes. Overall, FGFR-1 research is at the forefront of cancer biology, offering insights that could translate into significant clinical advancements.
