Analytical Data
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Gene name
FGFR-1
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简介
The FGFR-1 protein is a tyrosine-protein kinase receptor for fibroblast growth factors that is critical in embryonic development, cell proliferation, differentiation, and migration. GnRH is essential for mesodermal patterning, axial organization, skeletogenesis, and nervous system development. FGFR-1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived FGFR-1 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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Biological Activity
Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH 3T3 mouse fibroblast cells. The ED50 for this effect is 28.13ng/mL, corresponding to a specific activity is 35549.2357 Unit/mg. Measured by its ability to inhibit FGF acidic-dependent proliferation of NIH 3T3 mouse fibroblast cells.The ED50 for this effect is 28.13ng/mL, corresponding to a specific activity is 35549.2357 Unit/mg.
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Alternative Names
Fibroblast growth factor receptor 1; FGFR-1; BFGFR; FLT-2; CD331; HBGFR
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Species
Mouse
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Source
HEK293
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Tag
C-His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P16092
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Expression Region
R22-E376
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AA Sequence
RPAPTLPEQAQPWGVPVEVESLLVHPGDLLQLRCRLRDDVQSINWLRDGVQLVESNRTRITGEEVEVRDSIPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRRPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLE
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Protein Length
Extracellular Domain
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Molecular Weight
60-80 kDa.
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Fibroblast growth factor receptor 1 (FGFR-1) is a critical receptor in cell signaling pathways that regulate various biological processes, including cell proliferation, differentiation, and survival. Aberrations in FGFR-1 signaling are implicated in numerous diseases, particularly various cancers, where its overexpression or mutations can lead to tumorigenesis. Research on FGFR-1 has gained momentum due to its potential as a therapeutic target; inhibiting its activity may offer new avenues for cancer treatment. Recombinant FGFR-1 proteins are produced for functional studies to elucidate the receptor's mechanism of action and its interactions with ligands and downstream signaling molecules. Such studies are crucial for developing FGFR-1 inhibitors and understanding their efficacy in cancer therapy. Additionally, the characterization of FGFR-1 in different contexts, such as in the presence of tumor microenvironment factors, is essential for interpreting its role in disease progression. Through recombinant protein studies, researchers aim to develop selective antagonists and explore combination therapies to enhance treatment outcomes. Overall, FGFR-1 research is at the forefront of cancer biology, offering insights that could translate into significant clinical advancements.
