Analytical Data
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Gene name
IL-2
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简介
IL-2 Protein, produced by activated T-cells, NK cells, and helper T-cells, is crucial for immune response and tolerance. It binds to receptor complexes, activating downstream signaling pathways. IL-2 Protein acts as a growth factor, enhances NK-cell activity, promotes B-cell proliferation, and regulates regulatory T-cells. It also participates in differentiating effector T-cell subsets and memory CD8-positive T-cells. IL-2 Protein, Rat (145a.a, HEK293, C-His) is the recombinant rat-derived IL-2 protein, expressed by HEK293 , with C-10*His labeled tag.
- Application
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Biological Activity
Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×106 units/mg. Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.1884 ng/mL, corresponding to a specific activity is 5.307×106 units/mg.
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Alternative Names
Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin; IL2
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Species
Rat
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Source
HEK293
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Tag
C-10*His
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Purity
Greater than 95% as determined by reducing SDS-PAGE. as determined by SDS-PAGE.
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Uniprot
P17108
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Expression Region
Ala21~Gln155
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Protein Length
Partial
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Molecular Weight
18 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-2 (IL-2) is a pivotal cytokine in the immune system, primarily produced by T cells, and it plays a crucial role in the proliferation and activation of immune cells, particularly T lymphocytes. The recombinant form of IL-2 has gained significant attention in biomedical research and therapeutic applications due to its potential to enhance immune responses. Originally discovered for its capacity to support T cell growth, IL-2 has been researched extensively for its implications in cancer immunotherapy, autoimmune diseases, and transplant acceptance. The ability to produce IL-2 recombinantly has enabled scientists to explore its effects in a controlled manner, paving the way for the development of novel treatments aimed at boosting anti-tumor immunity or modulating excessive immune responses. Research involving IL-2 recombinant protein has led to a better understanding of its mechanisms, such as promoting T cell differentiation and memory formation, while also identifying optimal dosing strategies and delivery methods to maximize efficacy and minimize adverse effects. Furthermore, recombinant IL-2 has been integral in developing adoptive cell therapies, such as CAR T-cell therapy, where it is used to enhance the survival and functionality of engineered T cells. Overall, the study of IL-2 recombinant protein represents a cornerstone of immunotherapy research, highlighting its versatility and importance in manipulating the immune system for therapeutic benefit.
