Analytical Data
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Gene name
Aminopeptidase N/APN
- Application
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Alternative Names
CD13; ALAP; P150; ANPEP; APN; LAP1; PEPN; Gp150; Aminopeptidase M; Microsomal Aminopeptidase; Alanyl Membrane Aminopeptidase; Aminopeptidase N
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Species
Mouse
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Source
E. coli
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Tag
N-His
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Purity
Greater than 95% as determined by reducing SDS-PAGE.
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Uniprot
P97449
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Expression Region
Tyr720~Phe961
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Molecular Weight
30kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Aminopeptidase N (APN), also known as CD13, is a zinc-dependent metallopeptidase that plays a crucial role in various physiological processes, including protein metabolism and immune response regulation. APN is widely expressed on the surface of different cell types, including immune cells, endothelial cells, and some epithelial cells. It is involved in the degradation of extracellular peptides, thus influencing signaling pathways that are vital for cell proliferation, differentiation, and migration. In addition, APN has been implicated in the pathology of several diseases, including cancer, where it can affect tumor growth and metastasis, and in various infectious diseases, as it serves as a receptor for certain pathogens. The study of recombinant APN proteins has gained significant attention for their potential applications in diagnostics and therapeutics, particularly in designing inhibitors that can selectively target APN for cancer treatment or as a means to modulate immune responses. The ability to produce APN in a recombinant form allows for detailed characterization of its structure and function, paving the way for understanding its role in disease mechanisms and developing APN-based strategies for intervention. This research is critical for exploring novel approaches to combat diseases where APN is a key player, thereby highlighting its significance in both basic biology and clinical applications.
