Analytical Data
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Gene name
GJA8
- Application
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Alternative Names
GJA8; Gap junction alpha-8 protein; Connexin-50; Cx50; Lens fiber protein MP70
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P48165
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Expression Region
1-433aa
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AA Sequence
MGDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDFVCNTQQPGCENVCYDEAFPISHIRLWVLQIIFVSTPSLMYVGHAVHYVRMEEKRKSREAEELGQQAGTNGGPDQGSVKKSSGSKGTKKFRLEGTLLRTYICHIIFKTLFEVGFIVGHYFLYGFRILPLYRCSRWPCPNVVDCFVSRPTEKTIFILFMLSVASVSLFLNVMELGHLGLKGIRSALKRPVEQPLGEIPEKSLHSIAVSSIQKAKGYQLLEEEKIVSHYFPLTEVGMVETSPLPAKPFNQFEEKISTGPLGDLSRGYQETLPSYAQVGAQEVEGEGPPAEEGAEPEVGEKKEEAERLTTEEQEKVAVPEGEKVETPGVDKEGEKEEPQSEKVSKQGLPAEKTPSLCPELTTDDARPLSRLSKASSRARSDDLTV
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Molecular Weight
74.58 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
GJA8, also known as Gap Junction Protein Alpha 8, plays a crucial role in intercellular communication by forming gap junctions between adjacent cells, particularly in the lens and corneal tissues of the eye. Mutations in the GJA8 gene are linked to various ocular disorders, including congenital cataracts and other hereditary eye diseases. Due to its significant role in maintaining lens transparency and homeostasis, GJA8 has garnered attention in biomedical research, particularly in understanding its molecular mechanisms and the impact of its dysfunction on eye health. The study of GJA8 recombinant proteins allows researchers to explore the structure-function relationships and interactions with other proteins within the gap junctions. This research not only enhances our understanding of lens biology but also opens potential avenues for therapeutic interventions in hereditary eye disorders. As advancements in protein expression and purification technologies continue, the field is poised to uncover novel insights into GJA8's function and its implications in ocular pathologies, making it a vital target for future research and potential treatments in ophthalmology.
