Analytical Data
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基因名
IL-12 beta
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简介
IL-12 beta Protein, also known as natural killer cell stimulatory factor 2, is a common subunit (p40) of IL-12 and IL-23. IL-12 is a inflammatory factor expressed by activated macrophages, and involves in Th1-type immune response against cancer[1]. IL-12 beta Protein located outside the cell membrane, involves in singalling mediated by Jak-STAT[3]. IL-12 beta Protein consists of 328 amino acids (M1-S328) with a fibronectin type-III domain (237-328 a.a). IL-12 beta Protein, Human (I23-S328) is produced in HEK293 cells with tag free.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. Immobilized IL‑12 R beta 1 at 5 μg/ml (100 μL/well) can bind biotinylated Human IL‑12beta .The ED50 for this effect is 61.53 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized IL‑12 R beta 1 at 5 μg/mL (100 μL/well) can bind biotinylated Human IL‑12 beta .The ED50 for this effect is 61.53 ng/mL.
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别名
Interleukin-12 subunit beta; IL-12B; Cytotoxic lymphocyte maturation factor 40 kDa subunit; CLMF p40; IL-12 subunit p40; NK cell stimulatory factor chain 2; NKSF2; IL12B; NKSF2
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种属
Human
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表达系统
HEK293
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标签
Tag Free
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纯度
> 90% as determined by SDS-PAGE.
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蛋白编号
P29460
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表达区间
I23-S328
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氨基酸序列
IWELKKDVYVVELDWYPDAPGEMVVLTCDTPEEDGITWTLDQSSEVLGSGKTLTIQVKEFGDAGQYTCHKGGEVLSHSLLLLHKKEDGIWSTDILKDQKEPKNKTFLRCEAKNYSGRFTCWWLTTISTDLTFSVKSSRGSSDPQGVTCGAATLSAERVRGDNKEYEYSVECQEDSACPAAEESLPIEVMVDAVHKLKYENYTSSFFIRDIIKPDPPKNLQLKPLKNSRQVEVSWEYPDTWSTPHSYFSLTFCVQVQGKSKREKKDRVFTDKTSATVICRKNASISVRAQDRYYSSSWSEWASVPCS
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蛋白长度
Full Length of Mature Protein
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分子量
40-47 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Interleukin-12 (IL-12) is a pivotal cytokine that plays a crucial role in immune regulation and host defense against pathogens and tumors. IL-12 is a heterodimeric protein composed of two subunits, p35 and p40, encoded by separate genes. The p40 subunit, often referred to as IL-12 beta, has garnered significant research interest due to its involvement in immune responses. IL-12 promotes T cell differentiation into Th1 cells, enhances the production of interferon-gamma, and stimulates cytotoxic T lymphocytes and natural killer (NK) cell activity, making it essential for combating intracellular pathogens and malignancies. Dysfunctional IL-12 signaling is implicated in various autoimmune diseases and cancers, further highlighting its relevance in immunotherapy applications. Recombinant IL-12 beta protein has been developed for therapeutic purposes, including cancer vaccines and immune modulation strategies. Studies have demonstrated that delivering IL-12 beta can enhance anti-tumor immunity and improve treatment outcomes in preclinical and clinical settings. Consequently, ongoing research is focused on optimizing the production, delivery mechanisms, and therapeutic efficacy of recombinant IL-12 beta, aiming to harness its potential to improve cancer therapies and address unmet medical needs in immunological disorders.
