Analytical Data
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Gene name
UGT1A10
- Application
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Alternative Names
UGT1A10; GNT1; UGT1; UDP-glucuronosyltransferase 1A10; UGT1A10; UDP-glucuronosyltransferase 1-10; UDPGT 1-10; UGT1*10; UGT1-10; UGT1.10; UDP-glucuronosyltransferase 1-J; UGT-1J; UGT1J
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9HAW8
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Expression Region
1-530 aa
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AA Sequence
MARAGWTSPVPLCVCLLLTCGFAEAGKLLVVPMDGSHWFTMQSVVEKLILRGHEVVVVMPEVSWQLERSLNCTVKTYSTSYTLEDQNREFMVFAHAQWKARAQSIFSLLMSSSSGFLDLFFSHCRSLFNDRKLVEYLKESSFDAVFLDPFDTCGLIVAKYFSLPSVVFTRGIFCHHLEEGAQCPAPLSYVPNDLLGFSDAMTFKERVWNHIVHLEDHLFCQYLFRNALEIASEILQTPVTAYDLYSHTSIWLLRTDFVLDYPKPVMPNMIFIGGINCHQGKPLPMEFEAYINASGEHGIVVFSLGSMVSEIPEKKAMAIADALGKIPQTVLWRYTGTRPSNLANNTILVKWLPQNDLLGHPMTRAFITHAGSHGVYESICNGVPMVMMPLFGDQMDNAKRMETKGAGVTLNVLEMTSEDLENALKAVINDKSYKENIMRLSSLHKDRPVEPLDLAVFWVEFVMRHKGAPHLRPAAHDLTWYQYHSLDVIGFLLAVVLTVAFITFKCCAYGYRKCLGKKGRVKKAHKSKTH
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Molecular Weight
84.04 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
UGT1A10 is a member of the UDP-glucuronosyltransferase (UGT) family, which plays a crucial role in the metabolism and detoxification of various endogenous and exogenous compounds through the process of glucuronidation. This enzyme catalyzes the conjugation of glucuronic acid to a variety of substrates, including drugs, hormones, and environmental pollutants, thereby enhancing their solubility and facilitating their excretion from the body. Research into UGT1A10 has gained attention due to its potential implications in pharmacogenetics, where genetic polymorphisms can influence an individual's metabolic capacity and drug response. Furthermore, alterations in UGT1A10 activity may be associated with various diseases, including cancer, highlighting its importance in biomedical research. The expression and functional characterization of recombinant UGT1A10 protein provide valuable insights into its enzymatic properties, substrate specificity, and regulatory mechanisms. Given the rising significance of personalized medicine, understanding the role of UGT1A10 in drug metabolism and its variations among individuals could pave the way for more targeted and effective therapeutic approaches. This research endeavors to elucidate the structure-activity relationship of UGT1A10, establish its pharmacokinetic profiles, and explore its interactions with different substrates, thereby advancing our knowledge of metabolic pathways and improving drug design and safety profiles.
