Analytical Data
-
基因名
UBR3
- Application
-
别名
DKFZp434P117; DKFZp686N10185; E3 ubiquitin-Protein ligase UBR3; FLJ37422; KIAA2024; MGC126489; N-recognin-3; Ubiquitin-Protein ligase E3-alpha-3; Ubiquitin-Protein ligase E3-alpha-III; Ubr3; UBR3_HUMAN; Zinc finger Protein 650; ZNF 650; ZNF650
-
种属
Human
-
表达系统
E. coli
-
标签
GST-tag at N-terminal
-
纯度
Greater than 90% as determined by SDS-PAGE.
-
蛋白编号
Q6ZT12
-
表达区间
1-741 aa
-
氨基酸序列
MNKRIIEEICRKVTPPVPPKKVTAAEKKTLDKEERRQKARERQQKLLAEFASRQKSFMETAMDVDSPENDIPMEITTAEPQVSEAVYDCVICGQSGPSSEDRPTGLVVLLQASSVLGQCRDNVEPKKLPISEEEQIYPWDTCAAVHDVRLSLLQRYFKDSSCLLAVSIGWEGGVYVQTCGHTLHIDCHKSYMESLRNDQVLQGFSVDKGEFTCPLCRQFANSVLPCYPGSNVENNPWQRPSNKSIQDLIKEVEELQGRPGAFPSETNLSKEMESVMKDIKNTTQKKYRDYSKTPGSPDNDFLFMYSVARTNLELELIHRGGNLCSGGASTAGKRSCLNQLFHVLALHMRLYSIDSEYNPWRKLTQLEEMNPQLGYEEQQPEVPILYHDVTSLLLIQILMMPQPLRKDHFTCIVKVLFTLLYTQALAALSVKCSEEDRSAWKHAGALKKSTCDAEKSYEVLLSFVISELFKGKLYHEEGTQECAMVNPIAWSPESMEKCLQDFCLPFLRITSLLQHHLFGEDLPSCQEEEEFSVLASCLGLLPTFYQTEHPFISASCLDWPVPAFDIITQWCFEIKSFTERHAEQGKALLIQESKWKLPHLLQLPENYNTIFQYYHRKTCSVCTKVPKDPAVCLVCGTFVCLKGLCCKQQSYCECVLHSQNCGAGTGIFLLINASVIIIIRGHRFCLWGSVYLDAHGEEDRDLRRGKPLYICKERYKVLEQQWISHTFDHINKRWGPHYNGL
-
分子量
110.9 kDa
-
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
-
性状
Freeze-dried powder
-
缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
-
稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
UBR3, or Ubiquitin Protein Ligase E3 Component N-Recognin 3, is an important member of the E3 ubiquitin ligase family, which plays a critical role in the ubiquitin-proteasome system responsible for protein degradation and turnover within cells. This enzyme specifically recognizes and tags substrate proteins with ubiquitin molecules, thereby influencing various cellular processes such as cell cycle regulation, DNA repair, and signal transduction. Recent studies have indicated that UBR3 is implicated in several diseases, including cancer and neurodegenerative disorders, due to its role in the regulation of key oncoproteins and tumor suppressors. Research on UBR3 recombinant protein has become increasingly important for understanding its structure-function relationships and biological functions. The recombinant expression of UBR3 allows for detailed functional assays and structural studies, enabling scientists to dissect its mechanistic pathways and interactions with substrates. Furthermore, UBR3's potential as a therapeutic target in oncology underscores the necessity for in-depth investigations into its regulatory mechanisms. Consequently, the study of UBR3 not only enhances our understanding of cellular proteostasis but also paves the way for the development of innovative strategies aimed at modulating its activity for therapeutic benefits in disease contexts.
