Analytical Data
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Gene name
FBXO22
- Application
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Alternative Names
0610033L19Rik; 1600016C16Rik; F box only protein 22; F box protein 22; F box protein FBX22p44 ; F-box only protein 22; F-box protein FBX22p44; FBX22; FBX22_HUMAN; FBXO 22; FBXO22; FIST domain containing 1; FISTC1; FLJ13986; MGC124731; MGC31799
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q8NEZ5
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Expression Region
1-403aa
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AA Sequence
MEPVGCCGEC RGSSVDPRST FVLSNLAEVV ERVLTFLPAK ALLRVACVCR LWRECVRRVL RTHRSVTWIS AGLAEAGHLE GHCLVRVVAE ELENVRILPH TVLYMADSET FISLEECRGH KRARKRTSME TALALEKLFP KQCQVLGIVT PGIVVTPMGS GSNRPQEIEI GESGFALLFP QIEGIKIQPF HFIKDPKNLT LERHQLTEVG LLDNPELRVV LVFGYNCCKV GASNYLQQVV STFSDMNIIL AGGQVDNLSS LTSEKNPLDI DASGVVGLSF SGHRIQSATV LLNEDVSDEK TAEAAMQRLK AANIPEHNTI GFMFACVGRG FQYYRAKGNV EADAFRKFFP SVPLFGFFGN GEIGCDRIVT GNFILRKCNE VKDDDLFHSY TTIMALIHLG SSK
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Molecular Weight
44.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
FBXO22 is a member of the F-box protein family, which plays a crucial role in the ubiquitin-proteasome system, a key pathway for protein degradation in eukaryotic cells. F-box proteins, including FBXO22, serve as substrates for SCF (SKP1-Cullin-F-box) E3 ubiquitin ligase complexes, facilitating the ubiquitination and subsequent proteasomal degradation of specific target proteins. Research into FBXO22 has gained momentum due to its potential involvement in various biological processes, including cell cycle regulation, signal transduction, and cellular stress responses. Dysregulation of FBXO22 and its associated pathways has been implicated in several diseases, particularly cancer, where aberrant protein turnover can lead to uncontrolled cell proliferation. Additionally, studies have suggested that FBXO22 may interact with key oncogenic pathways, positioning it as a potential biomarker or therapeutic target in oncology. Given the complexity and significance of its roles, the recombinant expression and characterization of FBXO22 can provide valuable insights into its molecular functions and interactions, offering prospects for understanding its contributions to cellular homeostasis and disease mechanisms. Hence, investigating FBXO22, through recombinant protein studies, can pave the way for novel therapeutic interventions and enhance our comprehension of its role in health and disease.
