Analytical Data
-
Gene name
TRIM14
- Application
-
Alternative Names
5830400N10Rik ; KIAA0129; pub; TRI14_HUMAN; TRIM14; tripartite motif Protein 14; tripartite motif Protein TRIM14; tripartite motif-containing 14; Tripartite motif-containing Protein 14
-
Species
Human
-
Source
E. coli
-
Tag
His tag N-Terminus
-
Purity
Greater than 90% as determined by SDS-PAGE.
-
Uniprot
Q14142
-
Expression Region
1-442 aa
-
AA Sequence
MAGAATGSRT PGRSELVEGC GWRCPEHGDR VAELFCRRCR RCVCALCPVL GAHRGHPVGL ALEAAVHVQK LSQECLKQLA IKKQQHIDNI TQIEDATEKL KANAESSKTW LKGKFTELRL LLDEEEALAK KFIDKNTQLT LQVYREQADS CREQLDIMND LSNRVWSISQ EPDPVQRLQA YTATEQEMQQ QMSLGELCHP VPLSFEPVKS FFKGLVEAVE STLQTPLDIR LKESINCQLS DPSSTKPGTL LKTSPSPERS LLLKYARTPT LDPDTMHARL RLSADRLTVR CGLLGSLGPV PVLRFDALWQ VLARDCFATG RHYWEVDVQE AGAGWWVGAA YASLRRRGAS AAARLGCNRQ SWCLKRYDLE YWAFHDGQRS RLRPRDDLDR LGVFLDYEAG VLAFYDVTGG MSHLHTFRAT FQEPLYPALR LWEGAISIPR LP
-
Molecular Weight
49.7 kDa
-
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
-
Form
Freeze-dried powder
-
Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
-
Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
-
Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
-
Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
-
Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TRIM14, a member of the tripartite motif (TRIM) family of proteins, has garnered significant attention in recent years due to its implications in various biological processes, including immune response, cell proliferation, and apoptosis. This protein features a RING domain, which is characteristic of TRIM family members and is known to play a critical role in protein ubiquitination, thus regulating protein stability and function. The research surrounding TRIM14 has revealed its involvement in modulating inflammatory responses, particularly in the context of viral infections and autoimmune diseases. Additionally, studies have suggested that TRIM14 may influence various signaling pathways, including those related to NF-κB and interferon signaling, further underscoring its potential as a therapeutic target. However, the precise mechanisms through which TRIM14 exerts its effects remain unclear, prompting ongoing investigations into its role at the molecular level. Researchers are now focusing on generating recombinant TRIM14 proteins to facilitate these studies, which may provide insights into its structure-function relationships and interactions with other cellular components. By elucidating these aspects, scientists hope to uncover the therapeutic potential of TRIM14 in treating inflammatory conditions and other diseases linked to dysregulated immune responses, making it a promising area of study in molecular biology and immunology.
