Analytical Data
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Gene name
CAST
- Application
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Alternative Names
CAST；GATSL3；Cytosolic arginine sensor for mTORC1 subunit 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P20810
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Expression Region
1-686aa
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AA Sequence
MNPTETKAVKTEPEKKSQSTKLSVVHEKKSQEGKPKEHTEPKSLPKQASD TGSNDAHNKKAVSRSAEQQPSEKSTEPKTKPQDMISAGGESVAGITAISG KPGDKKKEKKSLTPAVPVESKPDKPSGKSGMDAALDDLIDTLGGPEETEE ENTTYTGPEVSDPMSSTYIEELGKREVTIPPKYRELLAKKEGITGPPADS SKPIGPDDAIDALSSDFTCGSPTAAGKKTEKEESTEVLKAQSAGTVRSAA PPQEKKRKVEKDTMSDQALEALSASLGTRQAEPELDLRSIKEVDEAKAKE EKLEKCGEDDETIPSEYRLKPATDKDGKPLLPEPEEKPKPRSESELIDEL SEDFDRSECKEKPSKPTEKTEESKAAAPAPVSEAVCRTSMCSIQSAPPEP ATLKGTVPDDAVEALADSLGKKEADPEDGKPVMDKVKEKAKEEDREKLGE KEETIPPDYRLEEVKDKDGKPLLPKESKEQLPPMSEDFLLDALSEDFSGP QNASSLKFEDAKLAAAISEVVSQTPASTTQAGAPPRDTSQSDKDLDDALD KLSDSLGQRQPDPDENKPMEDKVKEKAKAEHRDKLGERDDTIPPEYRHLL DDNGQDKPVKPPTKKSEDSKKPADDQDPIDALSGDLDSCPSTTETSQNTA KDKCKKAASSSKAPKNGGKAKDSAKTTEETSKPKDD
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Molecular Weight
102 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
CAST (Calcium/Calmodulin-dependent Serine Protein Kinase) is a crucial player in cellular signal transduction, influencing various physiological processes such as muscle contraction, neurotransmitter release, and gene expression. Its dysfunction has been linked to several pathological conditions, including heart disease, neurodegenerative disorders, and cancer. Given its significant role in these biological pathways, the study of CAST has gained considerable momentum, particularly in the context of developing targeted therapies and understanding the molecular mechanisms underpinning its activity. The recombinant expression of CAST proteins allows for detailed analysis of their structure and function, facilitating the identification of potential drug targets and therapeutic interventions. Investigating the post-translational modifications and interaction networks of CAST through recombinant techniques can elucidate its regulatory mechanisms, providing insights into how alterations in CAST function contribute to disease states. As researchers continue to explore the complexities of CAST and its implications in health and disease, the development of advanced biochemical tools for its manipulation and study remains vital to harness its potential in therapeutic applications. This research not only aims to deepen our understanding of CAST itself but also seeks to highlight its relevance in broader biological contexts.
