Analytical Data
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Gene name
PAOX
- Application
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Alternative Names
PAOX；PAO；Peroxisomal N(1)-acetyl-spermine/spermidine oxidase
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q6QHF9
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Expression Region
1-649aa
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AA Sequence
MESTGSVGEA PGGPRVLVVG GGIAGLGAAQ RLCGHSAFPH LRVLEATARA GGRIRSERCF GGVVEVGAHW IHGPSRGNPV FQLAAEYGLL GEKELSQENQ LVETGGHVGL PSVSYASSGT SVSLQLVAEM ATLFYGLIDQ TREFLHAAET PVPSVGEYLK KEIGQHVARL CGHSAFPHLR VLEATARAGG RIRSERCFGG VVEVGAHWIH GPSRGNPVFQ LAAEYGLLGE KELSQENQLV ETGGHVGLPS VSYASSGASV SLQLVAEMAT LFYGLIDQTR EFLHAAETPV PSVGEYLKKE IGQHVAGWTE DEETRKLKLA VLNSFFNLEC CVSGTHSMDL VALAPFGEYT VLPGLDCTFS KGYQGLTNCM MAALPEDTVV FEKPVKTIHW NGSFQEAAFP GETFPVSVEC EDGDRFPAHH VIVTVPLGFL REHLDTFFDP PLPAEKAEAI RKIGFGTNNK IFLEFEEPFW EPDCQLIQLV WEDTSPLEDA APELQDAWFR KLIGFVVLPA FASVHVLCGF IAGLESEFME TLSDEEVLLC LTQVLRRVTG NPRLPAPKSV LRSRWHSAPY TRGSYSYVAV GSTGGDLDLL AQPLPADGAG AQLQILFAGE ATHRTFYSTT HGALLSGWRE ADRLLSLWAP QVQQPRPRL
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PAOX, or polyamine oxidase, is an enzyme that plays a critical role in polyamine metabolism, which is essential for various cellular functions, including cell growth, differentiation, and apoptosis. Research into PAOX has gained momentum due to its implications in cancer, neurodegenerative diseases, and other pathological conditions. The enzyme catalyzes the oxidative degradation of polyamines, such as spermine and spermidine, producing hydrogen peroxide and other metabolites that can influence cellular signaling pathways. Understanding the structure and function of PAOX is crucial for deciphering its role in health and disease. Recent studies have focused on the recombinant expression of PAOX to facilitate detailed investigations of its enzymatic activity, regulatory mechanisms, and potential as a therapeutic target. By utilizing recombinant DNA technology, researchers can produce PAOX in larger quantities and investigate its properties in controlled settings, paving the way for the development of PAOX inhibitors or modulators that could have therapeutic applications. Overall, the exploration of PAOX as a critical regulator of polyamine homeostasis underscores its significance in cellular biology and its potential role in innovative therapeutic strategies.
