Analytical Data
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Gene name
Frizzled-4/CD344
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简介
Frizzled-4/CD344 is a Wnt receptor that mainly activates the β-catenin pathway and affects retinal vascularization. As a receptor for Wnt proteins and Norrin protein (NDP), it stimulates LEF/TCF-mediated transcriptional programs. Frizzled-4/CD344 Protein, Rat (HEK293, Fc) is the recombinant rat-derived Frizzled-4/CD344 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Alternative Names
CD344; EVR1; FEVR; Frizzled homolog 4 (Drosophila); Frizzled-4; Fz-4; FZD4
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Species
Rat
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9QZH0
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Expression Region
F38-E181
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Protein Length
Partial
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Molecular Weight
113 kDa & 58 kDa & 36 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-4 (FZD4), also known as CD344, is a member of the Frizzled family of G Protein-Coupled Receptors (GPCRs) that play a critical role in the Wnt signaling pathway, which is essential for various developmental processes and tissue homeostasis. Research on FZD4 has gained prominence due to its involvement in ocular diseases, particularly in the pathogenesis of diseases like retinopathy of prematurity (ROP) and age-related macular degeneration (AMD). FZD4 is known to mediate the effects of Wnt ligands, influencing cell proliferation, differentiation, and survival. Its role in angiogenesis and vascular development makes it a significant focus in understanding and potentially treating conditions characterized by abnormal blood vessel formation. Moreover, studies have indicated that mutations in FZD4 can lead to severe developmental disorders. The recombinant expression of FZD4/CD344 provides opportunities to explore its functional mechanisms, investigate ligand-receptor interactions, and assess its potential as a therapeutic target. Characterizing FZD4 at the molecular level also aids in deciphering its role in various signaling pathways, contributing to our overall understanding of cellular communication in health and disease.
