Analytical Data
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基因名
Frizzled-4/CD344
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简介
The Frizzled-4/CD344 protein is a receptor for Wnt proteins and is associated with the classical β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. In retinal vascularization, it acts as a receptor for Wnt proteins and Norrin (NDP), stimulating β-catenin accumulation and LEF/TCF-mediated transcription. Frizzled-4/CD344 Protein, Human (HEK293, His) is the recombinant human-derived Frizzled-4/CD344 protein, expressed by HEK293 , with C-His labeled tag.
- Application
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生物活性
Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-4, at 0.1 μg/mL (100 μL/well) can bind Wnt-5a. The ED50 for this effect is 26.59 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Human Frizzled-4, at 0.1 μg/mL (100 μL/well) can bind Wnt-5a. The ED50 for this effect is 26.59 ng/mL.
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别名
CD344 antigen; CD344; EVR1; FEVR; Frizzled-4; Fz-4; FZD4
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种属
Human
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表达系统
HEK293
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标签
C-His
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纯度
Greater than 95% as determined by reducing SDS-PAGE.
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蛋白编号
Q9ULV1
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表达区间
F37-E180
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蛋白长度
Partial
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分子量
22-25 kDa due to glycosylation
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
Frizzled-4 (FZD4), also known as CD344, is a member of the Frizzled family of G Protein-Coupled Receptors (GPCRs) that play a critical role in the Wnt signaling pathway, which is essential for various developmental processes and tissue homeostasis. Research on FZD4 has gained prominence due to its involvement in ocular diseases, particularly in the pathogenesis of diseases like retinopathy of prematurity (ROP) and age-related macular degeneration (AMD). FZD4 is known to mediate the effects of Wnt ligands, influencing cell proliferation, differentiation, and survival. Its role in angiogenesis and vascular development makes it a significant focus in understanding and potentially treating conditions characterized by abnormal blood vessel formation. Moreover, studies have indicated that mutations in FZD4 can lead to severe developmental disorders. The recombinant expression of FZD4/CD344 provides opportunities to explore its functional mechanisms, investigate ligand-receptor interactions, and assess its potential as a therapeutic target. Characterizing FZD4 at the molecular level also aids in deciphering its role in various signaling pathways, contributing to our overall understanding of cellular communication in health and disease.
