Analytical Data
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Gene name
Frizzled-4/CD344
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简介
The Frizzled-4/CD344 protein is a receptor for Wnt proteins and is associated with the classical β-catenin pathway, activating disheveled proteins, inhibiting GSK-3 kinase, and triggering Wnt target genes. In retinal vascularization, it acts as a receptor for Wnt proteins and Norrin (NDP), stimulating β-catenin accumulation and LEF/TCF-mediated transcription. Frizzled-4/CD344 Protein, Human (HEK293, Fc) is the recombinant human-derived Frizzled-4/CD344 protein, expressed by HEK293 , with C-hFc labeled tag.
- Application
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Biological Activity
Immobilized rmWnt-5a (HY-P704313) at 100 ng/mL (100 μL/well) can bind Frizzled-4/CD344, the ED50 for this effect is 1.740 μg/mL. Immobilized rmWnt-5a (HY-P704313) at 100 ng/mL (100 μL/well) can bind Frizzled-4/CD344, the ED50 for this effect is 1.740 μg/mL.
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Alternative Names
CD344 antigen; CD344; EVR1; FEVR; Frizzled-4; Fz-4; FZD4
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Species
Human
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Source
HEK293
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Tag
C-hFc
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9ULV1
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Expression Region
F37-E180
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Protein Length
Partial
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Molecular Weight
43.3 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
Frizzled-4 (FZD4), also known as CD344, is a member of the Frizzled family of G Protein-Coupled Receptors (GPCRs) that play a critical role in the Wnt signaling pathway, which is essential for various developmental processes and tissue homeostasis. Research on FZD4 has gained prominence due to its involvement in ocular diseases, particularly in the pathogenesis of diseases like retinopathy of prematurity (ROP) and age-related macular degeneration (AMD). FZD4 is known to mediate the effects of Wnt ligands, influencing cell proliferation, differentiation, and survival. Its role in angiogenesis and vascular development makes it a significant focus in understanding and potentially treating conditions characterized by abnormal blood vessel formation. Moreover, studies have indicated that mutations in FZD4 can lead to severe developmental disorders. The recombinant expression of FZD4/CD344 provides opportunities to explore its functional mechanisms, investigate ligand-receptor interactions, and assess its potential as a therapeutic target. Characterizing FZD4 at the molecular level also aids in deciphering its role in various signaling pathways, contributing to our overall understanding of cellular communication in health and disease.
