Analytical Data
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Gene name
DYNC1I2
- Application
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Alternative Names
Cytoplasmic dynein 1 intermediate chain 2; Cytoplasmic dynein intermediate chain 2; cytosolic; DC1I2_HUMAN; DH IC 2 ; DH IC-2; DNCI2 ; DNCIC2 ; Dync1i2
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q13409
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Expression Region
1-612aa
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AA Sequence
MSDKSELKAELERKKQRLAQIREEKKRKEEERKKKETDQKKEAVAPVQEESDLEKKRREAEALLQSMGLTPESPIVPPPMSPSSKSVSTPSEAGSQDSGDGAVGSRRGPIKLGMAKITQVDFPPREIVTYTKETQTPVMAQPKEDEEEDDDVVAPKPPIEPEEEKTLKKDEENDSKAPPHELTEEEKQQILHSEEFLSFFDHSTRIVERALSEQINIFFDYSGRDLEDKEGEIQAGAKLSLNRQFFDERWSKHRVVSCLDWSSQYPELLVASYNNNEDAPHEPDGVALVWNMKYKKTTPEYVFHCQSAVMSATFAKFHPNLVVGGTYSGQIVLWDNRSNKRTPVQRTPLSAAAHTHPVYCVNVVGTQNAHNLISISTDGKICSWSLDMLSHPQDSMELVHKQSKAVAVTSMSFPVGDVNNFVVGSEEGSVYTACRHGSKAGISEMFEGHQGPITGIHCHAAVGAVDFSHLFVTSSFDWTVKLWTTKNNKPLYSFEDNADYVYDVMWSPTHPALFACVDGMGRLDLWNLNNDTEVPTASISVEGNPALNRVRWTHSGREIAVGDSEGQIVIYDVGEQIAVPRNDEWARFGRTLAEINANRADAEEEAATRIPA
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Molecular Weight
94.8 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
DYNC1I2, a member of the dynein motor protein family, plays a crucial role in intracellular transport and organelle positioning. Research on DYNC1I2 has gained momentum due to its involvement in essential cellular processes such as mitosis, endocytosis, and the transport of organelles like lysosomes and mitochondria. Dysregulation of DYNC1I2 has been implicated in various neurodegenerative diseases, highlighting its potential as a therapeutic target. The recombinant expression of DYNC1I2 allows for detailed biochemical characterization, structure-function studies, and analysis of its interactions with other cellular components. This research is instrumental in understanding the mechanistic pathways of cellular transport and may lead to novel strategies for treating disorders associated with dynein dysfunction. As scientists explore the nuances of DYNC1I2's role in cellular dynamics, they also aim to uncover potential links to pathologies, paving the way for innovative therapeutic interventions.
