Analytical Data
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Gene name
TBP
- Application
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Alternative Names
TBP；GTF2D1；TF2D；TFIID；TATA-box-binding Protein
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P20226
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Expression Region
1-339aa
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AA Sequence
MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
TBP (TATA-binding protein) is a crucial component of the transcriptional machinery in eukaryotic cells, playing a central role in the initiation of gene expression by binding to the TATA box in the promoter region of genes. Its significance lies in its ability to facilitate the assembly of the transcriptional complex, influencing the regulation of numerous cellular processes. Research on recombinant TBP proteins has gained momentum due to their potential applications in understanding transcriptional regulation, mechanisms of gene expression, and cellular responses to environmental stimuli. Additionally, TBP is implicated in various diseases, including cancers and genetic disorders, making it a target for therapeutic interventions. The ability to produce TBP in a recombinant form allows for detailed biophysical and biochemical studies, providing insights into its structure-function relationships and interactions with other transcription factors and co-regulators. This research not only enhances our comprehension of fundamental biological processes but also opens avenues for drug discovery and the development of novel therapeutic strategies aimed at modulating gene expression in pathological conditions.
