Analytical Data
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基因名
TBP
- Application
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别名
TBP；GTF2D1；TF2D；TFIID；TATA-box-binding Protein
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P20226
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表达区间
1-339aa
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氨基酸序列
MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQQAVAAAAVQQSTSQQATQGTSGQAPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
TBP (TATA-binding protein) is a crucial component of the transcriptional machinery in eukaryotic cells, playing a central role in the initiation of gene expression by binding to the TATA box in the promoter region of genes. Its significance lies in its ability to facilitate the assembly of the transcriptional complex, influencing the regulation of numerous cellular processes. Research on recombinant TBP proteins has gained momentum due to their potential applications in understanding transcriptional regulation, mechanisms of gene expression, and cellular responses to environmental stimuli. Additionally, TBP is implicated in various diseases, including cancers and genetic disorders, making it a target for therapeutic interventions. The ability to produce TBP in a recombinant form allows for detailed biophysical and biochemical studies, providing insights into its structure-function relationships and interactions with other transcription factors and co-regulators. This research not only enhances our comprehension of fundamental biological processes but also opens avenues for drug discovery and the development of novel therapeutic strategies aimed at modulating gene expression in pathological conditions.
