Analytical Data
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基因名
SESN1
- Application
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别名
SESN1；PA26；SEST1；Sestrin-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9Y6P5-2
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表达区间
2-551aa
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氨基酸序列
AEGENEVRWDGLCSRDSTTRETALENIRQTILRKTEYLRSVKETPHRPSD GLSNTESSDGLNKLLAHLLMLSKRCPFKDVREKSEFILKSIQELGIRIPR PLGQGPSRFIPEKEILQVGSEDAQMHALFADSFAALGRLDNITLVMVFHP QYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVNDFL HVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEH SWSLAELVHAVVLLTHYHSLASFTFGCGISPEIHCDGGHTFRPPSVSNYC ICDITNGNHSVDEMPVNSAENVSVSDSFFEVEALMEKMRQLQECRDEEEA SQEEMASRFEIEKRESMFVFSSDDEEVTPARAVSRHFEDTSYGYKDFSRH GMHVPTFRVQDYCWEDHGYSLVNRLYPDVGQLIDEKFHIAYNLTYNTMAM HKDVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTVVC TPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRAITRYMT
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分子量
65 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SESN1 (Sestrin 1) is a highly conserved protein that plays a crucial role in cellular stress responses, metabolism, and the modulation of various signaling pathways, particularly in the context of oxidative stress and nutrient sensing. As a member of the Sestrin family, SESN1 is known to interact with key pathways such as mTOR (mechanistic Target of Rapamycin) and AMPK (AMP-activated Protein Kinase), which are vital for maintaining cellular homeostasis and energy balance. Given its protective functions against oxidative damage and its involvement in autophagy, SESN1 has garnered interest in research related to various diseases, including cancer, neurodegeneration, and metabolic disorders. The recombinant expression of SESN1 in heterologous systems has facilitated the investigation of its structural and functional properties, enabling researchers to explore its mechanism of action at a molecular level. As the understanding of SESN1's role in stress response and metabolism deepens, it holds potential as a therapeutic target, with implications for improving healthspan and treating age-related diseases. Current studies focus on elucidating the precise biochemical pathways impacted by SESN1, as well as the development of SESN1-based therapeutic strategies to combat conditions characterized by oxidative stress and cellular dysfunction. Overall, the research surrounding SESN1 recombinant protein continues to expand, contributing significantly to the fields of cell biology, biochemistry, and medical research.
