Analytical Data
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Gene name
SESN1
- Application
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Alternative Names
SESN1；PA26；SEST1；Sestrin-1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9Y6P5-2
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Expression Region
2-551aa
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AA Sequence
AEGENEVRWDGLCSRDSTTRETALENIRQTILRKTEYLRSVKETPHRPSD GLSNTESSDGLNKLLAHLLMLSKRCPFKDVREKSEFILKSIQELGIRIPR PLGQGPSRFIPEKEILQVGSEDAQMHALFADSFAALGRLDNITLVMVFHP QYLESFLKTQHYLLQMDGPLPLHYRHYIGIMAAARHQCSYLVNLHVNDFL HVGGDPKWLNGLENAPQKLQNLGELNKVLAHRPWLITKEHIEGLLKAEEH SWSLAELVHAVVLLTHYHSLASFTFGCGISPEIHCDGGHTFRPPSVSNYC ICDITNGNHSVDEMPVNSAENVSVSDSFFEVEALMEKMRQLQECRDEEEA SQEEMASRFEIEKRESMFVFSSDDEEVTPARAVSRHFEDTSYGYKDFSRH GMHVPTFRVQDYCWEDHGYSLVNRLYPDVGQLIDEKFHIAYNLTYNTMAM HKDVDTSMLRRAIWNYIHCMFGIRYDDYDYGEINQLLDRSFKVYIKTVVC TPEKVTKRMYDSFWRQFKHSEKVHVNLLLIEARMQAELLYALRAITRYMT
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Molecular Weight
65 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SESN1 (Sestrin 1) is a highly conserved protein that plays a crucial role in cellular stress responses, metabolism, and the modulation of various signaling pathways, particularly in the context of oxidative stress and nutrient sensing. As a member of the Sestrin family, SESN1 is known to interact with key pathways such as mTOR (mechanistic Target of Rapamycin) and AMPK (AMP-activated Protein Kinase), which are vital for maintaining cellular homeostasis and energy balance. Given its protective functions against oxidative damage and its involvement in autophagy, SESN1 has garnered interest in research related to various diseases, including cancer, neurodegeneration, and metabolic disorders. The recombinant expression of SESN1 in heterologous systems has facilitated the investigation of its structural and functional properties, enabling researchers to explore its mechanism of action at a molecular level. As the understanding of SESN1's role in stress response and metabolism deepens, it holds potential as a therapeutic target, with implications for improving healthspan and treating age-related diseases. Current studies focus on elucidating the precise biochemical pathways impacted by SESN1, as well as the development of SESN1-based therapeutic strategies to combat conditions characterized by oxidative stress and cellular dysfunction. Overall, the research surrounding SESN1 recombinant protein continues to expand, contributing significantly to the fields of cell biology, biochemistry, and medical research.
