Analytical Data
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Gene name
PRKAG2
- Application
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Alternative Names
PRKAG2；5'-AMP-activated Protein kinase subunit gamma-2
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
Q9UGJ0
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Expression Region
1-328aa
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AA Sequence
MLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETE
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Molecular Weight
41.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
Related Products
Protein Description
PRKAG2 encodes the AMP-activated protein kinase (AMPK) gamma-2 subunit, a critical component in cellular energy homeostasis and metabolic regulation. Mutations in the PRKAG2 gene have been associated with various pathological conditions, including familial hypertrophic cardiomyopathy, which presents a significant clinical challenge. Research has highlighted the role of AMPK in regulating key metabolic pathways, influencing glucose uptake, fatty acid oxidation, and overall energy balance within cells. Given its importance, recombinant PRKAG2 protein has garnered attention for its potential therapeutic applications in metabolic disorders. Scientists aim to better understand the functional implications of PRKAG2 mutations, the dynamics of AMPK signaling, and how these factors contribute to disease. The generation of recombinant PRKAG2 protein facilitates biochemical studies, enabling researchers to investigate the structure-function relationships of the gamma-2 subunit, including its interactions with other AMPK subunits and upstream kinases. By elucidating the molecular mechanisms underlying AMPK activation and the pathological consequences of PRKAG2 mutations, this research can pave the way for the development of targeted therapies aimed at restoring proper metabolic function and addressing associated diseases.
