Analytical Data
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基因名
SNF8
- Application
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别名
Dot3; ELL associated protein of 30 kDa; ELL-associated protein of 30 kDa; ESCRT-II complex subunit VPS22; hVps22; snf8; SNF8 ESCRTII complex subunit homolog (S cerevisiae); SNF8_HUMAN; Vacuolar sorting protein SNF8; Vacuolar-sorting protein SNF8; VPS22
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q96H20
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表达区间
1-258 aa
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氨基酸序列
MHRRGVGAGA IAKKKLAEAK YKERGTVLAE DQLAQMSKQL DMFKTNLEEF ASKHKQEIRK NPEFRVQFQD MCATIGVDPL ASGKGFWSEM LGVGDFYYEL GVQIIEVCLA LKHRNGGLIT LEELHQQVLK GRGKFAQDVS QDDLIRAIKK LKALGTGFGI IPVGGTYLIQ SVPAELNMDH TVVLQLAEKN GYVTVSEIKA SLKWETERAR QVLEHLLKEG LAWLDLQAPG EAHYWLPALF TDLYSQEITA EEAREALP
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分子量
28.8 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SNF8 is a crucial protein involved in the regulation of various cellular processes, including endocytosis and vesicle trafficking. It is a component of the ESCRT-III (endosomal sorting complex required for transport) complex, which plays a vital role in the sorting of ubiquitinated membrane proteins during the endosomal pathway. The study of SNF8 has gained significant attention due to its implications in maintaining cellular homeostasis and its potential link to various diseases, including cancer and neurodegenerative disorders. Researchers have focused on understanding the structural and functional characteristics of SNF8, as well as its interactions with other ESCRT components and cellular factors. The recombinant expression of SNF8 in heterologous systems has facilitated the functional analysis of this protein, enabling scientists to investigate its role in the dynamic processes of membrane remodeling and protein sorting. Furthermore, exploring the modulation of SNF8 activity may provide insights into therapeutic strategies for diseases associated with defects in endosomal trafficking. As such, SNF8 serves as a key target for further research in cell biology and biomedical applications, paving the way for a deeper understanding of its contributions to cellular physiology and pathology.
