Analytical Data
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基因名
PRKDC
- Application
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别名
PRKDC；HYRC；HYRC1；；DNA-dependent Protein kinase catalytic subunit
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P78527
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表达区间
3746-4128aa
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氨基酸序列
REHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVP MTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTK MSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPE AFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHA FGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDP GLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKIC YAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPE SGLSE ETQVKCLMDQATDPNILGRTWEGWEPWM
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分子量
68 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The PRKDC gene, encoding the protein DNA-dependent protein kinase catalytic subunit (DNA-PKcs), plays a crucial role in the DNA damage response and repair mechanisms, particularly through the non-homologous end joining (NHEJ) pathway. This pathway is vital for maintaining genomic stability, as it repairs double-strand breaks (DSBs) that can arise from various sources, including environmental factors and cellular processes. Dysregulation of DNA-PKcs has been implicated in various diseases, including cancer, where impaired DNA repair can lead to oncogenesis and tumor progression. Recent studies have focused on the recombinant expression of PRKDC to better understand its enzymatic functions and regulatory mechanisms. By producing recombinant DNA-PKcs in a controlled laboratory setting, researchers can investigate its structure, interaction with other proteins, and modulation of its activity. Additionally, targeted therapies that inhibit DNA-PKcs are being explored to enhance the efficacy of existing cancer treatments, as blocking this repair pathway may sensitize tumors to DNA-damaging agents. Understanding the role of recombinant PRKDC is pivotal for developing innovative therapeutic strategies and elucidating the underlying mechanisms of DNA repair in health and disease.
