Analytical Data
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Gene name
PRKDC
- Application
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Alternative Names
PRKDC；HYRC；HYRC1；；DNA-dependent Protein kinase catalytic subunit
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P78527
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Expression Region
3746-4128aa
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AA Sequence
REHPFLVKGGEDLRQDQRVEQLFQVMNGILAQDSACSQRALQLRTYSVVP MTSRLGLIEWLENTVTLKDLLLNTMSQEEKAAYLSDPRAPPCEYKDWLTK MSGKHDVGAYMLMYKGANRTETVTSFRKRESKVPADLLKRAFVRMSTSPE AFLALRSHFASSHALICISHWILGIGDRHLNNFMVAMETGGVIGIDFGHA FGSATQFLPVPELMPFRLTRQFINLMLPMKETGLMYSIMVHALRAFRSDP GLLTNTMDVFVKEPSFDWKNFEQKMLKKGGSWIQEINVAEKNWYPRQKIC YAKRKLAGANPAVITCDELLLGHEKAPAFRDYVAVARGSKDHNIRAQEPE SGLSE ETQVKCLMDQATDPNILGRTWEGWEPWM
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Molecular Weight
68 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
The PRKDC gene, encoding the protein DNA-dependent protein kinase catalytic subunit (DNA-PKcs), plays a crucial role in the DNA damage response and repair mechanisms, particularly through the non-homologous end joining (NHEJ) pathway. This pathway is vital for maintaining genomic stability, as it repairs double-strand breaks (DSBs) that can arise from various sources, including environmental factors and cellular processes. Dysregulation of DNA-PKcs has been implicated in various diseases, including cancer, where impaired DNA repair can lead to oncogenesis and tumor progression. Recent studies have focused on the recombinant expression of PRKDC to better understand its enzymatic functions and regulatory mechanisms. By producing recombinant DNA-PKcs in a controlled laboratory setting, researchers can investigate its structure, interaction with other proteins, and modulation of its activity. Additionally, targeted therapies that inhibit DNA-PKcs are being explored to enhance the efficacy of existing cancer treatments, as blocking this repair pathway may sensitize tumors to DNA-damaging agents. Understanding the role of recombinant PRKDC is pivotal for developing innovative therapeutic strategies and elucidating the underlying mechanisms of DNA repair in health and disease.
