Analytical Data
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Gene name
SLC2A4
- Application
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Alternative Names
insulin-responsive; Glucose transporter GLUT 4; Glucose transporter type 4; Glucose transporter type 4 insulin responsive; GLUT 4; GLUT-4; GLUT4; GTR4_HUMAN; Insulin responsive glucose transporter type 4; kug; SLC 2A4; SLC2A4; solute carrier family 2 (facilitated glucose transporter) member 4; Solute carrier family 2 member 4; Solute carrier family 2. facilitated glucose transporter member 4
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Species
Human
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Source
E. coli
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Tag
GST-tag at N-terminal
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
P14672
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Expression Region
1-509 aa
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AA Sequence
MPSGFQQIGSEDGEPPQQRVTGTLVLAVFSAVLGSLQFGYNIGVINAPQKVIEQSYNETWLGRQGPEGPSSIPPGTLTTLWALSVAIFSVGGMISSFLIGIISQWLGRKRAMLVNNVLAVLGGSLMGLANAAASYEMLILGRFLIGAYSGLTSGLVPMYVGEIAPTHLRGALGTLNQLAIVIGILIAQVLGLESLLGTASLWPLLLGLTVLPALLQLVLLPFCPESPRYLYIIQNLEGPARKSLKRLTGWADVSGVLAELKDEKRKLERERPLSLLQLLGSRTHRQPLIIAVVLQLSQQLSGINAVFYYSTSIFETAGVGQPAYATIGAGVVNTVFTLVSVLLVERAGRRTLHLLGLAGMCGCAILMTVALLLLERVPAMSYVSIVAIFGFVAFFEIGPGPIPWFIVAELFSQGPRPAAMAVAGFSNWTSNFIIGMGFQYVAEAMGPYVFLLFAVLLLGFFIFTFLRVPETRGRTFDQISAAFHRTPSLLEQEVKPSTELEYLGPDEND
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Molecular Weight
81.2 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SLC2A4, also known as GLUT4 (Glucose Transporter Type 4), is a critical protein involved in glucose metabolism and insulin signaling. It primarily functions in adipose tissue and skeletal muscle, facilitating glucose uptake in response to insulin stimulation. Dysregulation of GLUT4 is associated with metabolic disorders, including type 2 diabetes and obesity. Research on SLC2A4 recombinant protein is essential for understanding its molecular mechanisms and physiological roles. The study of GLUT4's structure and function can provide insights into its interactions with insulin and other signaling molecules, potentially leading to therapeutic strategies for enhancing glucose homeostasis in metabolic diseases. Furthermore, expressing and purifying SLC2A4 as a recombinant protein allows researchers to investigate its biochemical properties, such as substrate affinity and transporter kinetics, in a controlled environment. This research is crucial for developing drugs that can effectively target GLUT4, thus offering new avenues for treating insulin resistance and related conditions. Additionally, as scientists explore the mechanisms of GLUT4 translocation to the plasma membrane, it becomes evident that understanding its behavior under various physiological and pathological states can pave the way for innovative treatments aimed at improving glucose uptake and overall metabolic health.
