Analytical Data
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Gene name
SIP1
- Application
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Alternative Names
Component of gems 2; Gem (nuclear organelle) associated protein 2; Gem associated protein 2; GEMI2_HUMAN; Gemin-2; gemin2; SIP 1; SIP-1; SIP1; SIP1 delta; SIP1-delta; SMN interacting protein 1; SMN interacting protein 1 delta; SMN-interacting protein 1; Survival interacting protein 1; Survival of motor neuron protein interacting protein 1; Survival of motor neuron protein-interacting protein 1
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Species
Human
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Source
E. coli
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Tag
His tag N-Terminus
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Purity
Greater than 90% as determined by SDS-PAGE.
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Uniprot
O14893
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Expression Region
1-280 aa
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AA Sequence
MRRAELAGLK TMAWVPAESA VEELMPRLLP VEPCDLTEGF DPSVPPRTPQ EYLRRVQIEA AQCPDVVVAQ IDPKKLKRKQ SVNISLSGCQ PAPEGYSPTL QWQQQQVAQF STVRQNVNKH RSHWKSQQLD SNVTMPKSED EEGWKKFCLG EKLCADGAVG PATNESPGID YVQIGFPPLL SIVSRMNQAT VTSVLEYLSN WFGERDFTPE LGRWLYALLA CLEKPLLPEA HSLIRQLARR CSEVRLLVDS KDDERVPALN LLICLVSRYF DQRDLADEPS
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Molecular Weight
31.5 kDa
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Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
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Form
Freeze-dried powder
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Buffer formulation
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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Reconstitution
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- Customization
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Stability Test
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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Storage & Shelf Life
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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Shipping
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
Quality inspection process
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Protein Description
SIP1, also known as Smad-interacting protein 1, is a crucial regulator implicated in various biological processes, including embryonic development, cell proliferation, and differentiation, primarily through its interaction with the TGF-β signaling pathway. Research has shown that SIP1 plays a significant role in epithelial-mesenchymal transition (EMT), a process essential for developmental and cancerous contexts. Dysregulation of SIP1 expression has been linked to several diseases, particularly cancer metastasis, where it may promote invasive tumor characteristics. Understanding the function and mechanism of SIP1 is critical for elucidating its role in these pathological conditions. The development of recombinant SIP1 protein has become essential for studying its biological functions and interactions at the molecular level, paving the way for potential therapeutic applications. Characterizing SIP1 through recombinant methods allows researchers to analyze its structure, binding affinities, and effects on target proteins, thereby providing insights into its roles in signaling pathways. Moreover, investigating the post-translational modifications of SIP1 can further clarify its regulatory functions and interactions in cellular contexts. Ultimately, research surrounding SIP1 and its recombinant protein offers a promising avenue for advancing our understanding of TGF-β-related signaling and developing targeted strategies for cancer treatment and other diseases linked to SIP1 dysregulation.
